Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6873 | 3' | -46.6 | NC_001875.2 | + | 5340 | 0.66 | 0.999875 |
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Target: 5'- cCGAGU-GCgGGGCGGugcUGGUcaUUGACc -3' miRNA: 3'- -GCUCAuCGaCCCGCUu--AUCA--AAUUGu -5' |
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| 6873 | 3' | -46.6 | NC_001875.2 | + | 22901 | 0.66 | 0.999837 |
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Target: 5'- ----cAGUUGGGCGGAUuGGUcgGGCAg -3' miRNA: 3'- gcucaUCGACCCGCUUA-UCAaaUUGU- -5' |
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| 6873 | 3' | -46.6 | NC_001875.2 | + | 54302 | 0.66 | 0.999833 |
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Target: 5'- uCGaAGUAGCUGGGCucGUGcacggccGUggGGCAa -3' miRNA: 3'- -GC-UCAUCGACCCGcuUAU-------CAaaUUGU- -5' |
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| 6873 | 3' | -46.6 | NC_001875.2 | + | 126947 | 0.66 | 0.999783 |
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Target: 5'- gGAGcAGCUGcGGCGcgaguuaAAUAGUUUGcgccGCAg -3' miRNA: 3'- gCUCaUCGAC-CCGC-------UUAUCAAAU----UGU- -5' |
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| 6873 | 3' | -46.6 | NC_001875.2 | + | 116811 | 0.67 | 0.999561 |
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Target: 5'- gCGAGUugAGCUGGucUGGAUAGUUgucGGCAc -3' miRNA: 3'- -GCUCA--UCGACCc-GCUUAUCAAa--UUGU- -5' |
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| 6873 | 3' | -46.6 | NC_001875.2 | + | 93427 | 0.69 | 0.996932 |
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Target: 5'- aGAGgcGCUGcacGGCGAGUGcuucaucguguggacGUUUGACGa -3' miRNA: 3'- gCUCauCGAC---CCGCUUAU---------------CAAAUUGU- -5' |
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| 6873 | 3' | -46.6 | NC_001875.2 | + | 24176 | 0.7 | 0.991527 |
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Target: 5'- aGuGUAGUauucGGGCGggUgcGGUUUAGCGu -3' miRNA: 3'- gCuCAUCGa---CCCGCuuA--UCAAAUUGU- -5' |
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| 6873 | 3' | -46.6 | NC_001875.2 | + | 2838 | 1.11 | 0.015819 |
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Target: 5'- gCGAGUAGCUGGGCGAAUAGUUUAACAg -3' miRNA: 3'- -GCUCAUCGACCCGCUUAUCAAAUUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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