Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6876 | 3' | -53.9 | NC_001875.2 | + | 100501 | 0.66 | 0.940717 |
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Target: 5'- aAGACgacaugGUCGggCUGCGCGUUGaacUCGa -3' miRNA: 3'- gUCUGa-----CAGCuaGACGUGCAACc--GGC- -5' |
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| 6876 | 3' | -53.9 | NC_001875.2 | + | 59572 | 0.68 | 0.894627 |
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Target: 5'- uGGAUgUGUUGcgCUGCAgCG-UGGCCGa -3' miRNA: 3'- gUCUG-ACAGCuaGACGU-GCaACCGGC- -5' |
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| 6876 | 3' | -53.9 | NC_001875.2 | + | 68907 | 0.69 | 0.849996 |
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Target: 5'- -cGAgUGUCGAgucaacgagCUGUGCGagGGCCGg -3' miRNA: 3'- guCUgACAGCUa--------GACGUGCaaCCGGC- -5' |
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| 6876 | 3' | -53.9 | NC_001875.2 | + | 36332 | 0.7 | 0.807089 |
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Target: 5'- gGGGCUGUUGcgCgUGUACGc-GGCCGg -3' miRNA: 3'- gUCUGACAGCuaG-ACGUGCaaCCGGC- -5' |
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| 6876 | 3' | -53.9 | NC_001875.2 | + | 9680 | 0.71 | 0.750246 |
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Target: 5'- gCGGcGCUGUCGAaaaaauUgUGCGCGUgcacccaucgUGGCCGg -3' miRNA: 3'- -GUC-UGACAGCU------AgACGUGCA----------ACCGGC- -5' |
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| 6876 | 3' | -53.9 | NC_001875.2 | + | 72939 | 0.71 | 0.71005 |
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Target: 5'- --cGCUGUCGAUCUGCAgCGUuucgGGCg- -3' miRNA: 3'- gucUGACAGCUAGACGU-GCAa---CCGgc -5' |
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| 6876 | 3' | -53.9 | NC_001875.2 | + | 101554 | 0.72 | 0.699803 |
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Target: 5'- aCGGACgcguugCGAaacaUCUGCAaccCGUUGGCCGg -3' miRNA: 3'- -GUCUGaca---GCU----AGACGU---GCAACCGGC- -5' |
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| 6876 | 3' | -53.9 | NC_001875.2 | + | 99592 | 0.74 | 0.574827 |
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Target: 5'- gCGGGUUGUCGcgccgguauGUCUGCGCGUUGGCg- -3' miRNA: 3'- -GUCUGACAGC---------UAGACGUGCAACCGgc -5' |
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| 6876 | 3' | -53.9 | NC_001875.2 | + | 71975 | 0.75 | 0.523766 |
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Target: 5'- -cGAUUGUCGAacgugCgcaGCAUGUUGGCCGg -3' miRNA: 3'- guCUGACAGCUa----Ga--CGUGCAACCGGC- -5' |
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| 6876 | 3' | -53.9 | NC_001875.2 | + | 352 | 1.08 | 0.004675 |
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Target: 5'- gCAGACUGUCGAUCUGCACGUUGGCCGc -3' miRNA: 3'- -GUCUGACAGCUAGACGUGCAACCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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