Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6878 | 3' | -55.5 | NC_001875.2 | + | 90980 | 0.66 | 0.8794 |
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Target: 5'- aGGAUGgcGCCcgcGGUGcGCGUcuUG-GCGAg -3' miRNA: 3'- aCCUACauCGG---CCAC-CGCA--ACaCGUU- -5' |
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| 6878 | 3' | -55.5 | NC_001875.2 | + | 101962 | 0.67 | 0.799221 |
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Target: 5'- ----aGgcGCCGGUggcgaacgcgacgcaGGCGUUGUGCAu -3' miRNA: 3'- accuaCauCGGCCA---------------CCGCAACACGUu -5' |
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| 6878 | 3' | -55.5 | NC_001875.2 | + | 11818 | 0.69 | 0.70644 |
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Target: 5'- cGGuguuUGUGgucgcGCCGGUGGCGgggucgGUGUAAa -3' miRNA: 3'- aCCu---ACAU-----CGGCCACCGCaa----CACGUU- -5' |
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| 6878 | 3' | -55.5 | NC_001875.2 | + | 124193 | 0.69 | 0.696074 |
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Target: 5'- gUGGAUGUAGagCGcGUcGGCGUgUGUGCGc -3' miRNA: 3'- -ACCUACAUCg-GC-CA-CCGCA-ACACGUu -5' |
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| 6878 | 3' | -55.5 | NC_001875.2 | + | 56450 | 0.71 | 0.590841 |
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Target: 5'- -cGGUGaGGCCGGUGGCGggGUccGCGc -3' miRNA: 3'- acCUACaUCGGCCACCGCaaCA--CGUu -5' |
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| 6878 | 3' | -55.5 | NC_001875.2 | + | 552 | 1.06 | 0.003204 |
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Target: 5'- gUGGAUGUAGCCGGUGGCGUUGUGCAAg -3' miRNA: 3'- -ACCUACAUCGGCCACCGCAACACGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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