Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6879 | 5' | -56.3 | NC_001875.2 | + | 26220 | 0.71 | 0.535807 |
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Target: 5'- -cGGCGAC--CAGGGCCGCGUaCCGg- -3' miRNA: 3'- aaUUGCUGacGUCCCGGCGCA-GGUac -5' |
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| 6879 | 5' | -56.3 | NC_001875.2 | + | 57690 | 0.72 | 0.495887 |
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Target: 5'- cUGGCGGCgGCGGGcGCCGCGcCCu-- -3' miRNA: 3'- aAUUGCUGaCGUCC-CGGCGCaGGuac -5' |
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| 6879 | 5' | -56.3 | NC_001875.2 | + | 120743 | 0.72 | 0.495887 |
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Target: 5'- --uGCGGCgccgaGaCGGGGCCGUGUCCAg- -3' miRNA: 3'- aauUGCUGa----C-GUCCCGGCGCAGGUac -5' |
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| 6879 | 5' | -56.3 | NC_001875.2 | + | 110690 | 0.73 | 0.447988 |
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Target: 5'- -aAGCGGCcGCggaGGGcGCCGCGUUCGUGg -3' miRNA: 3'- aaUUGCUGaCG---UCC-CGGCGCAGGUAC- -5' |
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| 6879 | 5' | -56.3 | NC_001875.2 | + | 84171 | 0.73 | 0.438716 |
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Target: 5'- --cGCGGCgcGCcGcGGCCGCGUCCGUGc -3' miRNA: 3'- aauUGCUGa-CGuC-CCGGCGCAGGUAC- -5' |
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| 6879 | 5' | -56.3 | NC_001875.2 | + | 69249 | 0.74 | 0.411578 |
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Target: 5'- -gAACGGCgcGCuGGGCCGCGgcucgagCCGUGg -3' miRNA: 3'- aaUUGCUGa-CGuCCCGGCGCa------GGUAC- -5' |
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| 6879 | 5' | -56.3 | NC_001875.2 | + | 10863 | 0.8 | 0.182658 |
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Target: 5'- -gGACGAC-GCGGuGGCCGCGUCgGUGg -3' miRNA: 3'- aaUUGCUGaCGUC-CCGGCGCAGgUAC- -5' |
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| 6879 | 5' | -56.3 | NC_001875.2 | + | 922 | 1.06 | 0.002996 |
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Target: 5'- cUUAACGACUGCAGGGCCGCGUCCAUGa -3' miRNA: 3'- -AAUUGCUGACGUCCCGGCGCAGGUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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