Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 688 | 5' | -49.1 | AC_000018.1 | + | 21439 | 0.67 | 0.866599 |
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Target: 5'- gGCCuCGAugacgccGCGGugUGGCu-GGUGCa -3' miRNA: 3'- gCGG-GCUuu-----CGUUugACCGuuUCAUG- -5' |
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| 688 | 5' | -49.1 | AC_000018.1 | + | 16269 | 0.67 | 0.848498 |
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Target: 5'- gGCCCaAccGCGAAgaGGCAAuguGUACu -3' miRNA: 3'- gCGGGcUuuCGUUUgaCCGUUu--CAUG- -5' |
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| 688 | 5' | -49.1 | AC_000018.1 | + | 15574 | 0.68 | 0.816314 |
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Target: 5'- aCGUgCGuGAGCGAAgUGGCugucggagcugcugAGAGUACa -3' miRNA: 3'- -GCGgGCuUUCGUUUgACCG--------------UUUCAUG- -5' |
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| 688 | 5' | -49.1 | AC_000018.1 | + | 24776 | 0.68 | 0.788101 |
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Target: 5'- aCGCCucggaCGAGGGCAAGCcGGUAGucAGUGa -3' miRNA: 3'- -GCGG-----GCUUUCGUUUGaCCGUU--UCAUg -5' |
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| 688 | 5' | -49.1 | AC_000018.1 | + | 25149 | 0.7 | 0.697357 |
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Target: 5'- uGCCauaccuGGCAGACUGGCAuGGGUGu -3' miRNA: 3'- gCGGgcuu--UCGUUUGACCGU-UUCAUg -5' |
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| 688 | 5' | -49.1 | AC_000018.1 | + | 23661 | 0.74 | 0.474301 |
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Target: 5'- gGCCuUGGAAGCGgauGGCUGGCAGAGc-- -3' miRNA: 3'- gCGG-GCUUUCGU---UUGACCGUUUCaug -5' |
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| 688 | 5' | -49.1 | AC_000018.1 | + | 8462 | 0.76 | 0.37125 |
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Target: 5'- gGaCCCGgcGGCAuGGCUGGCAGuGGUACg -3' miRNA: 3'- gC-GGGCuuUCGU-UUGACCGUU-UCAUG- -5' |
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| 688 | 5' | -49.1 | AC_000018.1 | + | 18451 | 1.11 | 0.001695 |
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Target: 5'- gCGCCCGAAAGCAAACUGGCAAAGUACu -3' miRNA: 3'- -GCGGGCUUUCGUUUGACCGUUUCAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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