Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6880 | 5' | -56.9 | NC_001875.2 | + | 122073 | 0.66 | 0.779288 |
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Target: 5'- aUGuGCCGCGUGCGCUUGg-CCUcGGa -3' miRNA: 3'- gACuUGGCGUACGCGGACgaGGA-CUa -5' |
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| 6880 | 5' | -56.9 | NC_001875.2 | + | 111896 | 0.67 | 0.760048 |
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Target: 5'- -cGGACCGUgcGCGCCggccGCUCCg--- -3' miRNA: 3'- gaCUUGGCGuaCGCGGa---CGAGGacua -5' |
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| 6880 | 5' | -56.9 | NC_001875.2 | + | 46313 | 0.69 | 0.658317 |
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Target: 5'- aUGAACCGCGUGCugaucacgugGCCgccGCUUUUGGa -3' miRNA: 3'- gACUUGGCGUACG----------CGGa--CGAGGACUa -5' |
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| 6880 | 5' | -56.9 | NC_001875.2 | + | 81352 | 0.69 | 0.647868 |
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Target: 5'- gCUGGuGCCGCGcgcacGCGCCggccgcgucGCUCCUGAUu -3' miRNA: 3'- -GACU-UGGCGUa----CGCGGa--------CGAGGACUA- -5' |
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| 6880 | 5' | -56.9 | NC_001875.2 | + | 128099 | 0.69 | 0.647868 |
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Target: 5'- gUGGACCGCAUucacCGCUUGCagCUGAa -3' miRNA: 3'- gACUUGGCGUAc---GCGGACGagGACUa -5' |
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| 6880 | 5' | -56.9 | NC_001875.2 | + | 117294 | 0.7 | 0.574827 |
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Target: 5'- -cGAugGCCGCGUGCGCCaGCUCaCUc-- -3' miRNA: 3'- gaCU--UGGCGUACGCGGaCGAG-GAcua -5' |
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| 6880 | 5' | -56.9 | NC_001875.2 | + | 1199 | 1.05 | 0.002968 |
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Target: 5'- gCUGAACCGCAUGCGCCUGCUCCUGAUc -3' miRNA: 3'- -GACUUGGCGUACGCGGACGAGGACUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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