Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6888 | 3' | -53.9 | NC_001875.2 | + | 119432 | 0.7 | 0.727476 |
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Target: 5'- cUGCUUGgcgcGcGGCAAAC-AGCgGCCCGa -3' miRNA: 3'- cACGAACa---C-CCGUUUGuUCGaCGGGU- -5' |
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| 6888 | 3' | -53.9 | NC_001875.2 | + | 62415 | 0.7 | 0.727476 |
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Target: 5'- uUGC--GUGGGCAAGCGuuugcGCUGCgCCGg -3' miRNA: 3'- cACGaaCACCCGUUUGUu----CGACG-GGU- -5' |
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| 6888 | 3' | -53.9 | NC_001875.2 | + | 127893 | 0.71 | 0.685503 |
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Target: 5'- -gGUUUGUGGGCGcgcauuugAACAAGCggGCCg- -3' miRNA: 3'- caCGAACACCCGU--------UUGUUCGa-CGGgu -5' |
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| 6888 | 3' | -53.9 | NC_001875.2 | + | 47035 | 0.73 | 0.578513 |
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Target: 5'- -gGCggagugGUGGGCGugcccgacAACGGGCUGCUCAa -3' miRNA: 3'- caCGaa----CACCCGU--------UUGUUCGACGGGU- -5' |
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| 6888 | 3' | -53.9 | NC_001875.2 | + | 67223 | 0.74 | 0.51576 |
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Target: 5'- -cGCUgGUGGGCGcaAACGGGUgGCCCGa -3' miRNA: 3'- caCGAaCACCCGU--UUGUUCGaCGGGU- -5' |
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| 6888 | 3' | -53.9 | NC_001875.2 | + | 8783 | 0.93 | 0.03328 |
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Target: 5'- cGUGCUUG-GuGGCAAACAAGCUGCCCAg -3' miRNA: 3'- -CACGAACaC-CCGUUUGUUCGACGGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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