Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6892 | 3' | -50.4 | NC_001875.2 | + | 9692 | 0.67 | 0.990568 |
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Target: 5'- cCGuUGGGGcucGCCGUCGUgcgcgccgGCCGC-GCCGc -3' miRNA: 3'- -GC-ACUCU---UGGCAGCAa-------UGGUGaUGGU- -5' |
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| 6892 | 3' | -50.4 | NC_001875.2 | + | 57959 | 0.68 | 0.979942 |
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Target: 5'- uCGUGcGGGACCGcCGUUucgcgguuuucgcGCCGC-ACCAa -3' miRNA: 3'- -GCAC-UCUUGGCaGCAA-------------UGGUGaUGGU- -5' |
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| 6892 | 3' | -50.4 | NC_001875.2 | + | 76522 | 0.68 | 0.972527 |
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Target: 5'- gCGUcAucACCGUCGcgcGCCACUGCCGc -3' miRNA: 3'- -GCAcUcuUGGCAGCaa-UGGUGAUGGU- -5' |
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| 6892 | 3' | -50.4 | NC_001875.2 | + | 6934 | 0.7 | 0.951284 |
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Target: 5'- aCGUGAGcAACgCGUCGUgcgugcGCCGCgacaucGCCAc -3' miRNA: 3'- -GCACUC-UUG-GCAGCAa-----UGGUGa-----UGGU- -5' |
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| 6892 | 3' | -50.4 | NC_001875.2 | + | 97419 | 0.7 | 0.9469 |
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Target: 5'- gCGUGAauuugcgcaacGcGCCGUCGUUuuCCACUugCAu -3' miRNA: 3'- -GCACU-----------CuUGGCAGCAAu-GGUGAugGU- -5' |
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| 6892 | 3' | -50.4 | NC_001875.2 | + | 11597 | 1.1 | 0.008469 |
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Target: 5'- aCGUGAGAACCGUCGUUACCACUACCAc -3' miRNA: 3'- -GCACUCUUGGCAGCAAUGGUGAUGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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