miRNA: 3'- -UCCAACUaauACUCCuGUGCGCcGUU- -5'
miRNA: 3'- uccAACUaaua---CUCCUGUGCGCCGUU- -5'
miRNA: 3'- -UCCAACUAauaCUCCugUG-CGCCGUU- -5'
miRNA: 3'- -UCCAACUAAUACUCCUGUGCGCCGUU- -5'
Results 1 - 4 of 4 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6895 | 3' | -51.5 | NC_001875.2 | + | 56166 | 0.67 | 0.963869 |
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Target: 5'- cAGGUUGG---UGAGG-CACGCG-CAAa -3' miRNA: 3'- -UCCAACUaauACUCCuGUGCGCcGUU- -5' |
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| 6895 | 3' | -51.5 | NC_001875.2 | + | 5533 | 0.73 | 0.725867 |
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Target: 5'- --uUUGAcucggagGAGGACGCGCGGCAAu -3' miRNA: 3'- uccAACUaaua---CUCCUGUGCGCCGUU- -5' |
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| 6895 | 3' | -51.5 | NC_001875.2 | + | 73724 | 0.77 | 0.522696 |
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Target: 5'- gGGGUUGGUcggGAGGugGCuGCGGCGGu -3' miRNA: 3'- -UCCAACUAauaCUCCugUG-CGCCGUU- -5' |
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| 6895 | 3' | -51.5 | NC_001875.2 | + | 17229 | 1.06 | 0.008139 |
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Target: 5'- gAGGUUGAUUAUGAGGACACGCGGCAAc -3' miRNA: 3'- -UCCAACUAAUACUCCUGUGCGCCGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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