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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
690 | 5' | -55.7 | AC_000018.1 | + | 12109 | 0.66 | 0.550476 |
Target: 5'- cUCUGUcGCuCAGCUCGcGCCUccugcugGCGCUc -3' miRNA: 3'- -AGAUA-CG-GUUGAGC-CGGGua-----CGCGAc -5' |
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690 | 5' | -55.7 | AC_000018.1 | + | 16312 | 0.76 | 0.126367 |
Target: 5'- aCUAccgGCCAGCgCGuGCCCGUGCGCa- -3' miRNA: 3'- aGAUa--CGGUUGaGC-CGGGUACGCGac -5' |
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690 | 5' | -55.7 | AC_000018.1 | + | 21267 | 1.09 | 0.000395 |
Target: 5'- cUCUAUGCCAACUCGGCCCAUGCGCUGg -3' miRNA: 3'- -AGAUACGGUUGAGCCGGGUACGCGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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