Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6915 | 3' | -54.6 | NC_001875.2 | + | 63255 | 0.73 | 0.626772 |
Target: 5'- uCGGGCUcggcggccaGCGGCGCCAcgCGGUgcgcgaCCGc -3' miRNA: 3'- -GCUCGA---------UGCCGCGGUuaGCUAag----GGC- -5' |
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6915 | 3' | -54.6 | NC_001875.2 | + | 87842 | 0.73 | 0.616429 |
Target: 5'- uGGGC-ACGGCGCCcGUCGGgcugUCCGu -3' miRNA: 3'- gCUCGaUGCCGCGGuUAGCUaa--GGGC- -5' |
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6915 | 3' | -54.6 | NC_001875.2 | + | 5101 | 0.73 | 0.571162 |
Target: 5'- gGGGCUGCGcGCGCUAAUCGAcggaaaggucacgUCCgCGg -3' miRNA: 3'- gCUCGAUGC-CGCGGUUAGCUa------------AGG-GC- -5' |
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6915 | 3' | -54.6 | NC_001875.2 | + | 87268 | 0.74 | 0.524749 |
Target: 5'- gGAcGCcauUACGGCGCUAGUCGAUUcaaugcagCCCGg -3' miRNA: 3'- gCU-CG---AUGCCGCGGUUAGCUAA--------GGGC- -5' |
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6915 | 3' | -54.6 | NC_001875.2 | + | 32939 | 1.1 | 0.003352 |
Target: 5'- gCGAGCUACGGCGCCAAUCGAUUCCCGa -3' miRNA: 3'- -GCUCGAUGCCGCGGUUAGCUAAGGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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