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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 694 | 5' | -57.4 | AC_000018.1 | + | 24819 | 1.11 | 0.000235 |
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Target: 5'- aUCUCGCUGGCUGGGUACCAACUCCCCc -3' miRNA: 3'- -AGAGCGACCGACCCAUGGUUGAGGGG- -5' |
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| 694 | 5' | -57.4 | AC_000018.1 | + | 21944 | 0.71 | 0.238541 |
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Target: 5'- -gUUGCUGaCUGGGUGcCCAACagcaugcuccaaucUCCCCa -3' miRNA: 3'- agAGCGACcGACCCAU-GGUUG--------------AGGGG- -5' |
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| 694 | 5' | -57.4 | AC_000018.1 | + | 14519 | 0.69 | 0.311275 |
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Target: 5'- gUUCGUcaaaguugaUGGuCUGGGUGCUAGC-CUCCg -3' miRNA: 3'- aGAGCG---------ACC-GACCCAUGGUUGaGGGG- -5' |
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| 694 | 5' | -57.4 | AC_000018.1 | + | 5078 | 0.69 | 0.327215 |
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Target: 5'- gUUUCGCgGGUUGGGa--CGGCUCCUg -3' miRNA: 3'- -AGAGCGaCCGACCCaugGUUGAGGGg -5' |
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| 694 | 5' | -57.4 | AC_000018.1 | + | 5573 | 0.67 | 0.424396 |
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Target: 5'- -gUCGUguccccGCUGGGUGacaaagaggcuguCCGugUCCCCg -3' miRNA: 3'- agAGCGac----CGACCCAU-------------GGUugAGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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