Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6943 | 5' | -48 | NC_001875.2 | + | 12030 | 0.66 | 0.999135 |
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Target: 5'- -cGCuuUGAaaUCGAGGACGAGGAAAa--- -3' miRNA: 3'- cuCG--ACU--AGUUUCUGCUCCUUUacca -5' |
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| 6943 | 5' | -48 | NC_001875.2 | + | 19298 | 0.67 | 0.996709 |
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Target: 5'- -cGCUGcAUUGGacGGACGAGGggGUGa- -3' miRNA: 3'- cuCGAC-UAGUU--UCUGCUCCuuUACca -5' |
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| 6943 | 5' | -48 | NC_001875.2 | + | 125307 | 0.67 | 0.996709 |
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Target: 5'- cGAGUaugUGAcCAgcGACGAGGAccGGUGGa -3' miRNA: 3'- -CUCG---ACUaGUuuCUGCUCCU--UUACCa -5' |
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| 6943 | 5' | -48 | NC_001875.2 | + | 67165 | 0.7 | 0.981464 |
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Target: 5'- -uGCUGGaCGAggcGGGCGuGGAGAUGGa -3' miRNA: 3'- cuCGACUaGUU---UCUGCuCCUUUACCa -5' |
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| 6943 | 5' | -48 | NC_001875.2 | + | 97618 | 0.7 | 0.978159 |
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Target: 5'- gGGGCUGGcCAAuugccaacuguuuGACGAGGAgacAAUGGUg -3' miRNA: 3'- -CUCGACUaGUUu------------CUGCUCCU---UUACCA- -5' |
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| 6943 | 5' | -48 | NC_001875.2 | + | 21392 | 0.76 | 0.772734 |
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Target: 5'- aGAGCUGGUCAAAaacGACGAGGAGu---- -3' miRNA: 3'- -CUCGACUAGUUU---CUGCUCCUUuacca -5' |
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| 6943 | 5' | -48 | NC_001875.2 | + | 52428 | 1.09 | 0.012855 |
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Target: 5'- cGAGCUGAUCAAAGACGAGGAAAUGGUg -3' miRNA: 3'- -CUCGACUAGUUUCUGCUCCUUUACCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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