Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6952 | 5' | -52.7 | NC_001875.2 | + | 97539 | 0.72 | 0.714189 |
Target: 5'- -gGGCGCGuUUGGUGGCCAugGcugucGGGc -3' miRNA: 3'- aaUUGCGC-AACCACCGGUugCu----CUCa -5' |
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6952 | 5' | -52.7 | NC_001875.2 | + | 10156 | 0.87 | 0.121386 |
Target: 5'- -aAGCGCGgcgUGGUGGCCGACGAGAc- -3' miRNA: 3'- aaUUGCGCa--ACCACCGGUUGCUCUca -5' |
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6952 | 5' | -52.7 | NC_001875.2 | + | 62293 | 1.05 | 0.00812 |
Target: 5'- aUUAACGCGUUGGUGGCCAACGAGAGUu -3' miRNA: 3'- -AAUUGCGCAACCACCGGUUGCUCUCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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