Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6972 | 3' | -48.8 | NC_001875.2 | + | 82777 | 0.66 | 0.998345 |
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Target: 5'- uGCUgGGGAGGuCGUCGACcaGCguGAu -3' miRNA: 3'- uCGAgCUUUUU-GCAGCUGaaCGguCU- -5' |
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| 6972 | 3' | -48.8 | NC_001875.2 | + | 34857 | 0.66 | 0.99761 |
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Target: 5'- aAGCUgGAGucGAGCGUgugCGugcAUUUGCCGGAa -3' miRNA: 3'- -UCGAgCUU--UUUGCA---GC---UGAACGGUCU- -5' |
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| 6972 | 3' | -48.8 | NC_001875.2 | + | 80622 | 0.66 | 0.997151 |
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Target: 5'- gGGCgcgCGGAAcGCGcgcUCGACgggcggcGCCAGAa -3' miRNA: 3'- -UCGa--GCUUUuUGC---AGCUGaa-----CGGUCU- -5' |
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| 6972 | 3' | -48.8 | NC_001875.2 | + | 2276 | 0.66 | 0.997151 |
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Target: 5'- cGGCUCGAuacaauuacAAAACGcCGGCccGCCguGGAg -3' miRNA: 3'- -UCGAGCU---------UUUUGCaGCUGaaCGG--UCU- -5' |
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| 6972 | 3' | -48.8 | NC_001875.2 | + | 19966 | 0.67 | 0.996011 |
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Target: 5'- cGCUCGccAAAAugGUCaAUUUGCCcGAc -3' miRNA: 3'- uCGAGC--UUUUugCAGcUGAACGGuCU- -5' |
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| 6972 | 3' | -48.8 | NC_001875.2 | + | 62936 | 0.67 | 0.994523 |
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Target: 5'- cAGCUCGuu-GAC-UCGACacucGCCGGAc -3' miRNA: 3'- -UCGAGCuuuUUGcAGCUGaa--CGGUCU- -5' |
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| 6972 | 3' | -48.8 | NC_001875.2 | + | 32813 | 0.68 | 0.992615 |
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Target: 5'- aAGCUCGgcGAACGgCGccGCUUGCgAGc -3' miRNA: 3'- -UCGAGCuuUUUGCaGC--UGAACGgUCu -5' |
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| 6972 | 3' | -48.8 | NC_001875.2 | + | 48406 | 0.69 | 0.983602 |
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Target: 5'- cGGCUCGGGAgauuuacgggaaAACGUCGACggcaauuugcaUGCCGa- -3' miRNA: 3'- -UCGAGCUUU------------UUGCAGCUGa----------ACGGUcu -5' |
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| 6972 | 3' | -48.8 | NC_001875.2 | + | 46148 | 0.79 | 0.619354 |
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Target: 5'- cAGCUgGAucagauAAACGUCGACUUGUCGGc -3' miRNA: 3'- -UCGAgCUu-----UUUGCAGCUGAACGGUCu -5' |
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| 6972 | 3' | -48.8 | NC_001875.2 | + | 78764 | 1.1 | 0.011072 |
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Target: 5'- cAGCUCGAAAAACGUCGACUUGCCAGAc -3' miRNA: 3'- -UCGAGCUUUUUGCAGCUGAACGGUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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