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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6975 | 3' | -57.3 | NC_001875.2 | + | 9697 | 0.66 | 0.761164 |
Target: 5'- gGGGCUC-GCCGucGUGCGCGCCg---- -3' miRNA: 3'- -CUCGAGaCGGUc-UACGCGCGGaucau -5' |
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6975 | 3' | -57.3 | NC_001875.2 | + | 50962 | 0.7 | 0.544407 |
Target: 5'- -uGCUCUGCCAcucuuuGgcGCGCGCCgAGUc -3' miRNA: 3'- cuCGAGACGGU------CuaCGCGCGGaUCAu -5' |
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6975 | 3' | -57.3 | NC_001875.2 | + | 84293 | 1.07 | 0.001845 |
Target: 5'- cGAGCUCUGCCAGAUGCGCGCCUAGUAg -3' miRNA: 3'- -CUCGAGACGGUCUACGCGCGGAUCAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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