Results 61 - 64 of 64 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6977 | 3' | -54.5 | NC_001875.2 | + | 43693 | 0.66 | 0.943508 |
Target: 5'- cCGUCGUGCGCCcacacaauuUUGGcGC-CGCUCaGCa -3' miRNA: 3'- -GCAGCGCGUGG---------AACU-UGaGCGAGcUG- -5' |
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6977 | 3' | -54.5 | NC_001875.2 | + | 48196 | 0.66 | 0.943508 |
Target: 5'- -aUUGCGCACUUUGGGCaaaUGCccCGACa -3' miRNA: 3'- gcAGCGCGUGGAACUUGa--GCGa-GCUG- -5' |
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6977 | 3' | -54.5 | NC_001875.2 | + | 79805 | 0.66 | 0.943508 |
Target: 5'- uCGUCGgGC-CgCUUGAACgUGCcCGGCa -3' miRNA: 3'- -GCAGCgCGuG-GAACUUGaGCGaGCUG- -5' |
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6977 | 3' | -54.5 | NC_001875.2 | + | 41123 | 0.66 | 0.943508 |
Target: 5'- aCGUCGUGuCGCgCUUGAccgGCggccgCGC-CGGCa -3' miRNA: 3'- -GCAGCGC-GUG-GAACU---UGa----GCGaGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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