miRNA: 3'- gcAGCGCGUGgaACUUGAGCGaGCUG- -5'
miRNA: 3'- gCAGCG-CGUGG--AACUUGAGCGA-GC--UG- -5'
miRNA: 3'- -GCAG---CGCGUGGAACUUGaGCGA-GCUg -5'
miRNA: 3'- -GCAGCGCGUGGAACUUGAGCGAGCUG- -5'
Results 61 - 64 of 64 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6977 | 3' | -54.5 | NC_001875.2 | + | 54882 | 0.73 | 0.594422 |
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Target: 5'- -uUCGCGCAC--UGGGCUCGCaCGGCg -3' miRNA: 3'- gcAGCGCGUGgaACUUGAGCGaGCUG- -5' |
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| 6977 | 3' | -54.5 | NC_001875.2 | + | 56054 | 0.74 | 0.553367 |
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Target: 5'- uGUCGCgGCACUuuUUGAGCUCGCUgCGuuGCu -3' miRNA: 3'- gCAGCG-CGUGG--AACUUGAGCGA-GC--UG- -5' |
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| 6977 | 3' | -54.5 | NC_001875.2 | + | 59096 | 0.75 | 0.493561 |
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Target: 5'- gCGUUggaGCGCACCUUGGGC-CGCUaCGAg -3' miRNA: 3'- -GCAG---CGCGUGGAACUUGaGCGA-GCUg -5' |
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| 6977 | 3' | -54.5 | NC_001875.2 | + | 85151 | 1.11 | 0.002602 |
|
Target: 5'- aCGUCGCGCACCUUGAACUCGCUCGACg -3' miRNA: 3'- -GCAGCGCGUGGAACUUGAGCGAGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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