Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 6979 | 5' | -55.6 | NC_001875.2 | + | 110145 | 0.67 | 0.855703 |
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Target: 5'- gCUGGACCGcaGCACCGCGUcGcGCUc -3' miRNA: 3'- aGGUCUGGCa-CGUGGCGCAaCuUGGa -5' |
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| 6979 | 5' | -55.6 | NC_001875.2 | + | 111894 | 0.73 | 0.50501 |
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Target: 5'- gCCGGACCGUGCGcgccggccgcuCCGUGUacGGGCCg -3' miRNA: 3'- aGGUCUGGCACGU-----------GGCGCAa-CUUGGa -5' |
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| 6979 | 5' | -55.6 | NC_001875.2 | + | 112824 | 0.66 | 0.905314 |
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Target: 5'- gCCGcGCaCGUGCAaagCGCGUUGcGGCCUg -3' miRNA: 3'- aGGUcUG-GCACGUg--GCGCAAC-UUGGA- -5' |
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| 6979 | 5' | -55.6 | NC_001875.2 | + | 118323 | 0.67 | 0.855703 |
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Target: 5'- gCCGG-CCGcGCGCUGCagGUgaUGAACCUg -3' miRNA: 3'- aGGUCuGGCaCGUGGCG--CA--ACUUGGA- -5' |
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| 6979 | 5' | -55.6 | NC_001875.2 | + | 121483 | 0.66 | 0.885414 |
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Target: 5'- gCCAGGCCGUaGC-CCgGCGcaauUUGAAUCa -3' miRNA: 3'- aGGUCUGGCA-CGuGG-CGC----AACUUGGa -5' |
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| 6979 | 5' | -55.6 | NC_001875.2 | + | 124593 | 0.75 | 0.420701 |
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Target: 5'- gCCAGGCCGcUGCAgCGCGgaaagugcaUGAACCg -3' miRNA: 3'- aGGUCUGGC-ACGUgGCGCa--------ACUUGGa -5' |
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| 6979 | 5' | -55.6 | NC_001875.2 | + | 124773 | 0.66 | 0.905314 |
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Target: 5'- -aCAGGCCGgugGCgucGCCGCaGcgGAACCa -3' miRNA: 3'- agGUCUGGCa--CG---UGGCG-CaaCUUGGa -5' |
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| 6979 | 5' | -55.6 | NC_001875.2 | + | 127833 | 0.7 | 0.698822 |
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Target: 5'- gUCAGACgG-GCGCgGCGUUGGACg- -3' miRNA: 3'- aGGUCUGgCaCGUGgCGCAACUUGga -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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