Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6979 | 5' | -55.6 | NC_001875.2 | + | 109433 | 0.69 | 0.729022 |
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Target: 5'- -gCAGGCCGUGUACgCGCGgcaaaAGCCg -3' miRNA: 3'- agGUCUGGCACGUG-GCGCaac--UUGGa -5' |
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| 6979 | 5' | -55.6 | NC_001875.2 | + | 42902 | 0.7 | 0.708958 |
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Target: 5'- gUCgAGGCUGUGCGCCGCaugccgUGGGCg- -3' miRNA: 3'- -AGgUCUGGCACGUGGCGca----ACUUGga -5' |
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| 6979 | 5' | -55.6 | NC_001875.2 | + | 127833 | 0.7 | 0.698822 |
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Target: 5'- gUCAGACgG-GCGCgGCGUUGGACg- -3' miRNA: 3'- aGGUCUGgCaCGUGgCGCAACUUGga -5' |
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| 6979 | 5' | -55.6 | NC_001875.2 | + | 329 | 0.7 | 0.678386 |
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Target: 5'- uUCCAG-CCGcUGCACCGCGc---GCCg -3' miRNA: 3'- -AGGUCuGGC-ACGUGGCGCaacuUGGa -5' |
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| 6979 | 5' | -55.6 | NC_001875.2 | + | 12430 | 0.71 | 0.667076 |
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Target: 5'- -gCAGGCCGUugcgugcGCGCCGCGgUGGACg- -3' miRNA: 3'- agGUCUGGCA-------CGUGGCGCaACUUGga -5' |
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| 6979 | 5' | -55.6 | NC_001875.2 | + | 111894 | 0.73 | 0.50501 |
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Target: 5'- gCCGGACCGUGCGcgccggccgcuCCGUGUacGGGCCg -3' miRNA: 3'- aGGUCUGGCACGU-----------GGCGCAa-CUUGGa -5' |
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| 6979 | 5' | -55.6 | NC_001875.2 | + | 124593 | 0.75 | 0.420701 |
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Target: 5'- gCCAGGCCGcUGCAgCGCGgaaagugcaUGAACCg -3' miRNA: 3'- aGGUCUGGC-ACGUgGCGCa--------ACUUGGa -5' |
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| 6979 | 5' | -55.6 | NC_001875.2 | + | 87072 | 1.07 | 0.003447 |
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Target: 5'- cUCCAGACCGUGCACCGCGUUGAACCUc -3' miRNA: 3'- -AGGUCUGGCACGUGGCGCAACUUGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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