Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 698 | 3' | -57.8 | AC_000018.1 | + | 13133 | 0.66 | 0.467847 |
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Target: 5'- gCCG-CGCCCUGGg---UCAGGAAGa -3' miRNA: 3'- gGGCgGCGGGACCacauGGUCCUUUg -5' |
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| 698 | 3' | -57.8 | AC_000018.1 | + | 5270 | 0.67 | 0.409011 |
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Target: 5'- aUCGgCGCCCUGcaUGUcgGCCAGGuAGCa -3' miRNA: 3'- gGGCgGCGGGACc-ACA--UGGUCCuUUG- -5' |
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| 698 | 3' | -57.8 | AC_000018.1 | + | 21472 | 0.67 | 0.390447 |
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Target: 5'- gUUGCCGgCCgagaacGGUGUGCgCAGGuAGACg -3' miRNA: 3'- gGGCGGCgGGa-----CCACAUG-GUCC-UUUG- -5' |
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| 698 | 3' | -57.8 | AC_000018.1 | + | 21881 | 0.67 | 0.390447 |
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Target: 5'- cCCUGCaCGCUCUgGGUGgacuuuuCCAGGGugauGCa -3' miRNA: 3'- -GGGCG-GCGGGA-CCACau-----GGUCCUu---UG- -5' |
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| 698 | 3' | -57.8 | AC_000018.1 | + | 18105 | 0.68 | 0.346514 |
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Target: 5'- aCCCGCCGCCCgcaaccccUGGUGaaau-GGAAGu -3' miRNA: 3'- -GGGCGGCGGG--------ACCACaugguCCUUUg -5' |
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| 698 | 3' | -57.8 | AC_000018.1 | + | 17360 | 0.8 | 0.04973 |
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Target: 5'- uCCCGCCGCCCUGGUgcggagaguGUACCGcgauGGucGCg -3' miRNA: 3'- -GGGCGGCGGGACCA---------CAUGGU----CCuuUG- -5' |
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| 698 | 3' | -57.8 | AC_000018.1 | + | 27137 | 1.11 | 0.000246 |
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Target: 5'- gCCCGCCGCCCUGGUGUACCAGGAAACu -3' miRNA: 3'- -GGGCGGCGGGACCACAUGGUCCUUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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