Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6981 | 5' | -49.2 | NC_001875.2 | + | 104596 | 0.66 | 0.996103 |
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Target: 5'- --cUGCucgAAAGcGUCGCUUUgauacCCUCGUCg -3' miRNA: 3'- uuuGCG---UUUCuCAGCGAAA-----GGAGCAG- -5' |
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| 6981 | 5' | -49.2 | NC_001875.2 | + | 22482 | 0.67 | 0.992725 |
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Target: 5'- cGAACGCcacGGGG-CGCgUUUCCUUGUUc -3' miRNA: 3'- -UUUGCGuu-UCUCaGCG-AAAGGAGCAG- -5' |
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| 6981 | 5' | -49.2 | NC_001875.2 | + | 112313 | 0.67 | 0.990323 |
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Target: 5'- uAGACGCGuacguguuGAGUUGCUUg-CUUGUCa -3' miRNA: 3'- -UUUGCGUuu------CUCAGCGAAagGAGCAG- -5' |
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| 6981 | 5' | -49.2 | NC_001875.2 | + | 55338 | 0.68 | 0.98542 |
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Target: 5'- uGACGCGccgcuucGGGGG-CGCcg-CCUCGUCg -3' miRNA: 3'- uUUGCGU-------UUCUCaGCGaaaGGAGCAG- -5' |
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| 6981 | 5' | -49.2 | NC_001875.2 | + | 44154 | 0.68 | 0.976798 |
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Target: 5'- cAGCGCAcGucGUCGCUcUCCUCGa- -3' miRNA: 3'- uUUGCGUuUcuCAGCGAaAGGAGCag -5' |
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| 6981 | 5' | -49.2 | NC_001875.2 | + | 64898 | 0.68 | 0.976798 |
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Target: 5'- cGACGCucAGcuGUCGCg--CCUCGUUg -3' miRNA: 3'- uUUGCGuuUCu-CAGCGaaaGGAGCAG- -5' |
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| 6981 | 5' | -49.2 | NC_001875.2 | + | 65571 | 0.7 | 0.956996 |
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Target: 5'- uAAACGCAAAGGGgggaUCGUUgUCaauaUCGUCg -3' miRNA: 3'- -UUUGCGUUUCUC----AGCGAaAGg---AGCAG- -5' |
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| 6981 | 5' | -49.2 | NC_001875.2 | + | 98604 | 0.74 | 0.794801 |
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Target: 5'- cAAACGCAAAGAG-CGCg--CCaCGUCg -3' miRNA: 3'- -UUUGCGUUUCUCaGCGaaaGGaGCAG- -5' |
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| 6981 | 5' | -49.2 | NC_001875.2 | + | 88311 | 1.08 | 0.011441 |
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Target: 5'- aAAACGCAAAGAGUCGCUUUCCUCGUCu -3' miRNA: 3'- -UUUGCGUUUCUCAGCGAAAGGAGCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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