Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7001 | 5' | -61.9 | NC_001875.2 | + | 33551 | 0.69 | 0.411236 |
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Target: 5'- cGUUGGCCACagUG-CGGUCGCCgcggGGCa -3' miRNA: 3'- -CAGCCGGUGggACuGCCAGUGG----CCGa -5' |
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| 7001 | 5' | -61.9 | NC_001875.2 | + | 121192 | 0.7 | 0.387274 |
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Target: 5'- aGUCcGCCGCCCaaguacugcacgcugGGCGG-CACCGGCc -3' miRNA: 3'- -CAGcCGGUGGGa--------------CUGCCaGUGGCCGa -5' |
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| 7001 | 5' | -61.9 | NC_001875.2 | + | 115872 | 0.7 | 0.378428 |
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Target: 5'- -gCGGCCGCUCUGgccgggcaggauGCGGUCaaaguGCUGGCg -3' miRNA: 3'- caGCCGGUGGGAC------------UGCCAG-----UGGCCGa -5' |
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| 7001 | 5' | -61.9 | NC_001875.2 | + | 65435 | 0.7 | 0.370506 |
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Target: 5'- -cCGGCgugCACCgCUGGCGGUCGgUGGCc -3' miRNA: 3'- caGCCG---GUGG-GACUGCCAGUgGCCGa -5' |
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| 7001 | 5' | -61.9 | NC_001875.2 | + | 114492 | 0.71 | 0.347425 |
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Target: 5'- -gCGGCagCACUCUGacGCGGUCGCgCGGCg -3' miRNA: 3'- caGCCG--GUGGGAC--UGCCAGUG-GCCGa -5' |
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| 7001 | 5' | -61.9 | NC_001875.2 | + | 102018 | 0.71 | 0.339964 |
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Target: 5'- cGUCGGCCguGCCCgaGAaauauuuGUCGCCGGCg -3' miRNA: 3'- -CAGCCGG--UGGGa-CUgc-----CAGUGGCCGa -5' |
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| 7001 | 5' | -61.9 | NC_001875.2 | + | 45184 | 0.76 | 0.171683 |
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Target: 5'- uGUCGGCCGCCCUGACcacgcucgauucggGGcuUCAagaCGGCUg -3' miRNA: 3'- -CAGCCGGUGGGACUG--------------CC--AGUg--GCCGA- -5' |
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| 7001 | 5' | -61.9 | NC_001875.2 | + | 103016 | 1.06 | 0.001186 |
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Target: 5'- uGUCGGCCACCCUGACGGUCACCGGCUc -3' miRNA: 3'- -CAGCCGGUGGGACUGCCAGUGGCCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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