Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7010 | 3' | -50.6 | NC_001875.2 | + | 27973 | 0.66 | 0.993806 |
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Target: 5'- -gGCUUUUUAAGGAcCaAGGCGcgcGCGAg -3' miRNA: 3'- caUGAGAAGUUCCUuGcUCCGC---UGCU- -5' |
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| 7010 | 3' | -50.6 | NC_001875.2 | + | 65081 | 0.66 | 0.992835 |
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Target: 5'- -cACgCUgggCAAGGAcgcgcucaACGAGGCGGCc- -3' miRNA: 3'- caUGaGAa--GUUCCU--------UGCUCCGCUGcu -5' |
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| 7010 | 3' | -50.6 | NC_001875.2 | + | 99885 | 0.66 | 0.990528 |
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Target: 5'- cUGCg--UCAAGGuaaaacCGAGGCGcACGAa -3' miRNA: 3'- cAUGagaAGUUCCuu----GCUCCGC-UGCU- -5' |
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| 7010 | 3' | -50.6 | NC_001875.2 | + | 18428 | 0.66 | 0.989032 |
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Target: 5'- cGUACUCggcgCGAGGcGCGGuccgcguGGCGcGCGAa -3' miRNA: 3'- -CAUGAGaa--GUUCCuUGCU-------CCGC-UGCU- -5' |
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| 7010 | 3' | -50.6 | NC_001875.2 | + | 105589 | 0.67 | 0.982207 |
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Target: 5'- uGUGCgg--CAAGG-ACGAGGCGuACGc -3' miRNA: 3'- -CAUGagaaGUUCCuUGCUCCGC-UGCu -5' |
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| 7010 | 3' | -50.6 | NC_001875.2 | + | 84428 | 0.68 | 0.975087 |
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Target: 5'- gGUGCcaUCggCGgaucGGGAGCGGcGGCGGCGGu -3' miRNA: 3'- -CAUG--AGaaGU----UCCUUGCU-CCGCUGCU- -5' |
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| 7010 | 3' | -50.6 | NC_001875.2 | + | 102820 | 0.68 | 0.975087 |
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Target: 5'- cUGCUCgUCAAGGGccGCGAcuuugugcagcgGGUGAUGAc -3' miRNA: 3'- cAUGAGaAGUUCCU--UGCU------------CCGCUGCU- -5' |
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| 7010 | 3' | -50.6 | NC_001875.2 | + | 50332 | 0.69 | 0.941698 |
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Target: 5'- --uUUgUUCAGGGAcgugACG-GGCGACGAg -3' miRNA: 3'- cauGAgAAGUUCCU----UGCuCCGCUGCU- -5' |
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| 7010 | 3' | -50.6 | NC_001875.2 | + | 30910 | 0.71 | 0.901596 |
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Target: 5'- cGUAgaCgUUguGGGAGCGAGcGCGGCGAg -3' miRNA: 3'- -CAUgaG-AAguUCCUUGCUC-CGCUGCU- -5' |
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| 7010 | 3' | -50.6 | NC_001875.2 | + | 113327 | 1.1 | 0.007511 |
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Target: 5'- cGUACUCUUCAAGGAACGAGGCGACGAu -3' miRNA: 3'- -CAUGAGAAGUUCCUUGCUCCGCUGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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