Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7013 | 5' | -60.2 | NC_001875.2 | + | 59566 | 0.66 | 0.651623 |
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Target: 5'- aUUCGGUggaUGUguugCGCUGCagCGUGGCCGAc -3' miRNA: 3'- -AAGUCAg--ACA----GCGACG--GCGCCGGCUa -5' |
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| 7013 | 5' | -60.2 | NC_001875.2 | + | 4924 | 0.66 | 0.61063 |
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Target: 5'- -gUAGUCgGUCGUcaCCGCGGCCGu- -3' miRNA: 3'- aaGUCAGaCAGCGacGGCGCCGGCua -5' |
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| 7013 | 5' | -60.2 | NC_001875.2 | + | 105722 | 0.67 | 0.5902 |
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Target: 5'- cUCucgCUGUCGCUGCCccacUGGUCGAUg -3' miRNA: 3'- aAGucaGACAGCGACGGc---GCCGGCUA- -5' |
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| 7013 | 5' | -60.2 | NC_001875.2 | + | 117183 | 0.67 | 0.580027 |
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Target: 5'- -cCAGUUUGUgGCUGCa--GGCCGGc -3' miRNA: 3'- aaGUCAGACAgCGACGgcgCCGGCUa -5' |
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| 7013 | 5' | -60.2 | NC_001875.2 | + | 100143 | 0.7 | 0.400029 |
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Target: 5'- uUUguGUCUGcCGCUGUCGCGGUgaucaCGGUg -3' miRNA: 3'- -AAguCAGACaGCGACGGCGCCG-----GCUA- -5' |
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| 7013 | 5' | -60.2 | NC_001875.2 | + | 117259 | 1.03 | 0.001883 |
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Target: 5'- gUUCAGUCUGUCGCUGCCGCGGCCGAUg -3' miRNA: 3'- -AAGUCAGACAGCGACGGCGCCGGCUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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