Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7014 | 3' | -54.4 | NC_001875.2 | + | 19951 | 0.68 | 0.853024 |
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Target: 5'- gGCCgGCGCGAcgGUCCGGcCGGCGc-- -3' miRNA: 3'- -UGGgCGUGCUaaCAGGCU-GUUGCcuc -5' |
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| 7014 | 3' | -54.4 | NC_001875.2 | + | 78478 | 0.68 | 0.853024 |
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Target: 5'- uGCgCCGCAguuugaacaGGUUGUCggugGACGACGGAGa -3' miRNA: 3'- -UG-GGCGUg--------CUAACAGg---CUGUUGCCUC- -5' |
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| 7014 | 3' | -54.4 | NC_001875.2 | + | 69692 | 0.7 | 0.801965 |
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Target: 5'- --gCGCGCGAUUGUuuGACGucAUGGAc -3' miRNA: 3'- uggGCGUGCUAACAggCUGU--UGCCUc -5' |
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| 7014 | 3' | -54.4 | NC_001875.2 | + | 70167 | 0.71 | 0.72344 |
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Target: 5'- gGCCCGCugGAgcaUUGUCCGAaaaguguagucaAGCGGc- -3' miRNA: 3'- -UGGGCGugCU---AACAGGCUg-----------UUGCCuc -5' |
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| 7014 | 3' | -54.4 | NC_001875.2 | + | 97920 | 0.71 | 0.705294 |
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Target: 5'- cGCCCGC-UGcgUGUUCGACGAgGGGu -3' miRNA: 3'- -UGGGCGuGCuaACAGGCUGUUgCCUc -5' |
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| 7014 | 3' | -54.4 | NC_001875.2 | + | 5369 | 0.79 | 0.334531 |
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Target: 5'- cACCCGCugGAcgUGUUCGGugaGACGGAGg -3' miRNA: 3'- -UGGGCGugCUa-ACAGGCUg--UUGCCUC- -5' |
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| 7014 | 3' | -54.4 | NC_001875.2 | + | 118057 | 1.09 | 0.003895 |
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Target: 5'- cACCCGCACGAUUGUCCGACAACGGAGa -3' miRNA: 3'- -UGGGCGUGCUAACAGGCUGUUGCCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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