Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7017 | 3' | -57.1 | NC_001875.2 | + | 66116 | 0.66 | 0.805754 |
Target: 5'- gGCgGGCGgcgaGGCGGCGCcgcccgcggaacaguGGUCGGc -3' miRNA: 3'- -UGgUCGCa---CCGCCGCGauuu-----------CCAGUC- -5' |
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7017 | 3' | -57.1 | NC_001875.2 | + | 51932 | 0.66 | 0.806648 |
Target: 5'- uGCaCAGCGU-GCGGCGCUAcuuuGGGcgCGa -3' miRNA: 3'- -UG-GUCGCAcCGCCGCGAUu---UCCa-GUc -5' |
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7017 | 3' | -57.1 | NC_001875.2 | + | 3154 | 0.66 | 0.806648 |
Target: 5'- gACgCGGCGUcgGGCGGCGagu-AGGUgAGc -3' miRNA: 3'- -UG-GUCGCA--CCGCCGCgauuUCCAgUC- -5' |
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7017 | 3' | -57.1 | NC_001875.2 | + | 67169 | 0.66 | 0.824175 |
Target: 5'- -aCAGCGUGGUGGacacCGC----GGUCGGg -3' miRNA: 3'- ugGUCGCACCGCC----GCGauuuCCAGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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