Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7030 | 3' | -55.5 | NC_001876.1 | + | 4785 | 0.66 | 0.572111 |
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Target: 5'- cGGUGACGgucgaUGGCccgcaGGGUGCGGucuUUCCa -3' miRNA: 3'- -UUACUGC-----AUCG-----CCCACGCCuu-GAGGc -5' |
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| 7030 | 3' | -55.5 | NC_001876.1 | + | 3273 | 0.66 | 0.526426 |
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Target: 5'- --aGACGggcucgaacauguUGGgGGGUGUGGuACUCCc -3' miRNA: 3'- uuaCUGC-------------AUCgCCCACGCCuUGAGGc -5' |
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| 7030 | 3' | -55.5 | NC_001876.1 | + | 9035 | 0.67 | 0.505713 |
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Target: 5'- -cUGACcgaacuGCGGG-GCGGAgcuuuugaGCUCCGa -3' miRNA: 3'- uuACUGcau---CGCCCaCGCCU--------UGAGGC- -5' |
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| 7030 | 3' | -55.5 | NC_001876.1 | + | 25509 | 0.67 | 0.463325 |
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Target: 5'- uGAUGGCGcUGGCcuuuGGGgcgGCGGGGCgcCCGa -3' miRNA: 3'- -UUACUGC-AUCG----CCCa--CGCCUUGa-GGC- -5' |
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| 7030 | 3' | -55.5 | NC_001876.1 | + | 178 | 0.68 | 0.422873 |
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Target: 5'- --cGGCGcgcggGGCGGGgcgagggGCGGAGUUCCGc -3' miRNA: 3'- uuaCUGCa----UCGCCCa------CGCCUUGAGGC- -5' |
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| 7030 | 3' | -55.5 | NC_001876.1 | + | 97 | 0.68 | 0.413094 |
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Target: 5'- -cUGACGUAacuGUGGG-GCGGAGCgcgUCGc -3' miRNA: 3'- uuACUGCAU---CGCCCaCGCCUUGa--GGC- -5' |
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| 7030 | 3' | -55.5 | NC_001876.1 | + | 34350 | 0.68 | 0.413094 |
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Target: 5'- -cUGACGUAacuGUGGG-GCGGAGCgcgUCGc -3' miRNA: 3'- uuACUGCAU---CGCCCaCGCCUUGa--GGC- -5' |
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| 7030 | 3' | -55.5 | NC_001876.1 | + | 262 | 1.06 | 0.000863 |
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Target: 5'- aAAUGACGUAGCGGGUGCGGAACUCCGc -3' miRNA: 3'- -UUACUGCAUCGCCCACGCCUUGAGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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