Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7031 | 5' | -45.5 | NC_001876.1 | + | 34276 | 0.66 | 0.98323 |
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Target: 5'- cGCGggGCGGCgcgCGGGgcggggCAAAGGGc -3' miRNA: 3'- uCGCuuCGUCGaa-GUUUag----GUUUUCU- -5' |
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| 7031 | 5' | -45.5 | NC_001876.1 | + | 12447 | 0.66 | 0.98323 |
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Target: 5'- gGGCGGcacgccGGCGGCUUCGGcgCCc----- -3' miRNA: 3'- -UCGCU------UCGUCGAAGUUuaGGuuuucu -5' |
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| 7031 | 5' | -45.5 | NC_001876.1 | + | 9025 | 0.66 | 0.98323 |
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Target: 5'- uGCGggGCggAGCUUUuGAgcUCCGAccccGAGAa -3' miRNA: 3'- uCGCuuCG--UCGAAGuUU--AGGUU----UUCU- -5' |
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| 7031 | 5' | -45.5 | NC_001876.1 | + | 6490 | 0.68 | 0.953045 |
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Target: 5'- cAGUGggGCAGCccCAcugacgugugaacaaAGUCCAAauAAGAu -3' miRNA: 3'- -UCGCuuCGUCGaaGU---------------UUAGGUU--UUCU- -5' |
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| 7031 | 5' | -45.5 | NC_001876.1 | + | 1524 | 0.69 | 0.921852 |
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Target: 5'- uAGCGggG--GCUUCAAAUCgCAcuGGAg -3' miRNA: 3'- -UCGCuuCguCGAAGUUUAG-GUuuUCU- -5' |
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| 7031 | 5' | -45.5 | NC_001876.1 | + | 25401 | 0.69 | 0.921852 |
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Target: 5'- uGGCGGuuuuGGuCAGCUUCGGGUCUGcaguAAGAa -3' miRNA: 3'- -UCGCU----UC-GUCGAAGUUUAGGUu---UUCU- -5' |
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| 7031 | 5' | -45.5 | NC_001876.1 | + | 22823 | 0.7 | 0.883917 |
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Target: 5'- cGCGGuAGCAGCagCGGAgCCAGAAGc -3' miRNA: 3'- uCGCU-UCGUCGaaGUUUaGGUUUUCu -5' |
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| 7031 | 5' | -45.5 | NC_001876.1 | + | 30671 | 0.71 | 0.816353 |
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Target: 5'- gGGCGGuggaaggcaaaaaAGCAGCUUUGGAagCAGAGGGg -3' miRNA: 3'- -UCGCU-------------UCGUCGAAGUUUagGUUUUCU- -5' |
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| 7031 | 5' | -45.5 | NC_001876.1 | + | 22891 | 0.74 | 0.702849 |
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Target: 5'- cGCGAAGCGGCUUCuggcUCCGc---- -3' miRNA: 3'- uCGCUUCGUCGAAGuuu-AGGUuuucu -5' |
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| 7031 | 5' | -45.5 | NC_001876.1 | + | 1551 | 1.11 | 0.003656 |
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Target: 5'- gAGCGAAGCAGCUUCAAAUCCAAAAGAa -3' miRNA: 3'- -UCGCUUCGUCGAAGUUUAGGUUUUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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