Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7034 | 5' | -56.2 | NC_001876.1 | + | 14791 | 0.66 | 0.509022 |
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Target: 5'- cGCGCggcCGCUa-UC-GCAG-CAGACGCg -3' miRNA: 3'- -CGCGa--GCGAccAGuCGUCuGUCUGCG- -5' |
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| 7034 | 5' | -56.2 | NC_001876.1 | + | 16308 | 0.68 | 0.416875 |
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Target: 5'- aGCGCaCGCcGGgCAGCAGcgccGCGGcCGCg -3' miRNA: 3'- -CGCGaGCGaCCaGUCGUC----UGUCuGCG- -5' |
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| 7034 | 5' | -56.2 | NC_001876.1 | + | 6010 | 0.69 | 0.361395 |
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Target: 5'- aGUGuCUCGUUGGUcCAGCAGA--GGCGa -3' miRNA: 3'- -CGC-GAGCGACCA-GUCGUCUguCUGCg -5' |
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| 7034 | 5' | -56.2 | NC_001876.1 | + | 12304 | 0.73 | 0.186141 |
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Target: 5'- cGCaGCUCGCUGGaaauaguccaucagUCGguuuauaaagucGCGGugGGACGCg -3' miRNA: 3'- -CG-CGAGCGACC--------------AGU------------CGUCugUCUGCG- -5' |
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| 7034 | 5' | -56.2 | NC_001876.1 | + | 16233 | 0.74 | 0.158735 |
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Target: 5'- gGCGg-CGCccGGaCGGCGGGCAGGCGCa -3' miRNA: 3'- -CGCgaGCGa-CCaGUCGUCUGUCUGCG- -5' |
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| 7034 | 5' | -56.2 | NC_001876.1 | + | 4935 | 1.14 | 0.000187 |
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Target: 5'- gGCGCUCGCUGGUCAGCAGACAGACGCa -3' miRNA: 3'- -CGCGAGCGACCAGUCGUCUGUCUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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