Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7035 | 3' | -55.3 | NC_001876.1 | + | 24880 | 0.66 | 0.531621 |
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Target: 5'- uGUCUguGGC-GGCAGCUGuuGg---- -3' miRNA: 3'- -CAGAguCCGuUCGUCGACggCgaaug -5' |
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| 7035 | 3' | -55.3 | NC_001876.1 | + | 16569 | 0.66 | 0.509234 |
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Target: 5'- ----aAGGCGcgcgGGCGGCgGCCGCcUGCg -3' miRNA: 3'- cagagUCCGU----UCGUCGaCGGCGaAUG- -5' |
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| 7035 | 3' | -55.3 | NC_001876.1 | + | 4747 | 0.66 | 0.498189 |
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Target: 5'- uUCUUGGGUugGGGUGGCUGuuGCUg-- -3' miRNA: 3'- cAGAGUCCG--UUCGUCGACggCGAaug -5' |
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| 7035 | 3' | -55.3 | NC_001876.1 | + | 16752 | 0.67 | 0.434403 |
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Target: 5'- cUCUC-GGCcuGCAacuuCUGCUGCUUACg -3' miRNA: 3'- cAGAGuCCGuuCGUc---GACGGCGAAUG- -5' |
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| 7035 | 3' | -55.3 | NC_001876.1 | + | 22417 | 0.68 | 0.398477 |
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Target: 5'- aUCUUcGGCGGGgGGCUcagcgaucggcgcgcGCCGUUUGCg -3' miRNA: 3'- cAGAGuCCGUUCgUCGA---------------CGGCGAAUG- -5' |
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| 7035 | 3' | -55.3 | NC_001876.1 | + | 24809 | 0.68 | 0.366346 |
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Target: 5'- -gCUCAGGCuucggaGGGCcaacAGCUGCCGCcacagACa -3' miRNA: 3'- caGAGUCCG------UUCG----UCGACGGCGaa---UG- -5' |
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| 7035 | 3' | -55.3 | NC_001876.1 | + | 11086 | 0.7 | 0.274343 |
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Target: 5'- -aCUCGGGCcccggcaAAGCGGCcGCCGCggucGCg -3' miRNA: 3'- caGAGUCCG-------UUCGUCGaCGGCGaa--UG- -5' |
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| 7035 | 3' | -55.3 | NC_001876.1 | + | 6842 | 0.93 | 0.00586 |
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Target: 5'- aGUCUCAGGaacGCAGCUGCCGCUUACg -3' miRNA: 3'- -CAGAGUCCguuCGUCGACGGCGAAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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