Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7036 | 3' | -62.9 | NC_001876.1 | + | 15769 | 0.66 | 0.245784 |
Target: 5'- cAUCugCACUUCgGCCacggagcCGGCCCCG-Cg -3' miRNA: 3'- -UGGugGUGGAGgCGGa------GUCGGGGCaG- -5' |
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7036 | 3' | -62.9 | NC_001876.1 | + | 11140 | 0.66 | 0.233454 |
Target: 5'- aGCCAUgGCCgaagcgCCccgaaGCCUgGGCCCCGcCc -3' miRNA: 3'- -UGGUGgUGGa-----GG-----CGGAgUCGGGGCaG- -5' |
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7036 | 3' | -62.9 | NC_001876.1 | + | 22652 | 0.66 | 0.233454 |
Target: 5'- aGCCcuaGgCGCggCCGCUUCGGCCCCcUCa -3' miRNA: 3'- -UGG---UgGUGgaGGCGGAGUCGGGGcAG- -5' |
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7036 | 3' | -62.9 | NC_001876.1 | + | 16202 | 0.66 | 0.223968 |
Target: 5'- cGCCGCCGuacccgcagggcgacUCcCCGCCUCAGCgCUGcCg -3' miRNA: 3'- -UGGUGGU---------------GGaGGCGGAGUCGgGGCaG- -5' |
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7036 | 3' | -62.9 | NC_001876.1 | + | 8437 | 0.66 | 0.221648 |
Target: 5'- -gCGCCAUCUUCGUCUCGGgCUCG-Ca -3' miRNA: 3'- ugGUGGUGGAGGCGGAGUCgGGGCaG- -5' |
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7036 | 3' | -62.9 | NC_001876.1 | + | 26883 | 0.67 | 0.194346 |
Target: 5'- aGCCGCuCAcCCUCCGCgUCucCCCCG-Cg -3' miRNA: 3'- -UGGUG-GU-GGAGGCGgAGucGGGGCaG- -5' |
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7036 | 3' | -62.9 | NC_001876.1 | + | 22397 | 0.67 | 0.189251 |
Target: 5'- gGCCGCCGCUcCCGCCcugccggCGGCCagCCGg- -3' miRNA: 3'- -UGGUGGUGGaGGCGGa------GUCGG--GGCag -5' |
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7036 | 3' | -62.9 | NC_001876.1 | + | 12498 | 0.68 | 0.170032 |
Target: 5'- aGCCGCCGCgUgCCGCCcaCAGCUCCu-- -3' miRNA: 3'- -UGGUGGUGgA-GGCGGa-GUCGGGGcag -5' |
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7036 | 3' | -62.9 | NC_001876.1 | + | 14682 | 0.72 | 0.078133 |
Target: 5'- cACCACCGCCUCgauUGUgUCGGCCaCCGg- -3' miRNA: 3'- -UGGUGGUGGAG---GCGgAGUCGG-GGCag -5' |
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7036 | 3' | -62.9 | NC_001876.1 | + | 12491 | 0.73 | 0.071737 |
Target: 5'- cACCACCGCCgCCGCC-CGGUgCCG-Cg -3' miRNA: 3'- -UGGUGGUGGaGGCGGaGUCGgGGCaG- -5' |
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7036 | 3' | -62.9 | NC_001876.1 | + | 34274 | 0.77 | 0.031055 |
Target: 5'- cGCCGCCGCCUCCGCgacgcgCUCcGCCCCa-- -3' miRNA: 3'- -UGGUGGUGGAGGCG------GAGuCGGGGcag -5' |
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7036 | 3' | -62.9 | NC_001876.1 | + | 173 | 0.77 | 0.031055 |
Target: 5'- cGCCGCCGCCUCCGCgacgcgCUCcGCCCCa-- -3' miRNA: 3'- -UGGUGGUGGAGGCG------GAGuCGGGGcag -5' |
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7036 | 3' | -62.9 | NC_001876.1 | + | 34243 | 0.79 | 0.02319 |
Target: 5'- cGCCGCCcCCgCCGcCCUCAGCCCCGcCc -3' miRNA: 3'- -UGGUGGuGGaGGC-GGAGUCGGGGCaG- -5' |
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7036 | 3' | -62.9 | NC_001876.1 | + | 204 | 0.79 | 0.02319 |
Target: 5'- cGCCGCCcCCgCCGcCCUCAGCCCCGcCc -3' miRNA: 3'- -UGGUGGuGGaGGC-GGAGUCGGGGCaG- -5' |
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7036 | 3' | -62.9 | NC_001876.1 | + | 8561 | 1.08 | 0.00011 |
Target: 5'- cACCACCACCUCCGCCUCAGCCCCGUCu -3' miRNA: 3'- -UGGUGGUGGAGGCGGAGUCGGGGCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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