miRNA: 3'- aCCuCGa----UUUGAACCCGGUCCa--CCag -5'
miRNA: 3'- -ACCUCGAUUugaACCCGGu--CCaCCag -5'
miRNA: 3'- -ACCU----CGAUUUGaaCCCGgUCCA-CCAG- -5'
miRNA: 3'- aCCUcGAUUUGa-ACCCG-GUCCACCAG- -5'
miRNA: 3'- -ACCUCGAUUUGAACCCGGUCCAcCAG- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7036 | 5' | -54.7 | NC_001876.1 | + | 29417 | 0.68 | 0.459848 |
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Target: 5'- aGG-GCguuggAAGCUUGGGUCAGGccgGGa- -3' miRNA: 3'- aCCuCGa----UUUGAACCCGGUCCa--CCag -5' |
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| 7036 | 5' | -54.7 | NC_001876.1 | + | 6451 | 0.69 | 0.370936 |
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Target: 5'- uUGGAGCUGAuggUGGGCCuuuGGaGGa- -3' miRNA: 3'- -ACCUCGAUUugaACCCGGu--CCaCCag -5' |
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| 7036 | 5' | -54.7 | NC_001876.1 | + | 24466 | 0.7 | 0.335363 |
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Target: 5'- uUGGGucucGCUGAGCa-GGGCgGGGUuGGUCg -3' miRNA: 3'- -ACCU----CGAUUUGaaCCCGgUCCA-CCAG- -5' |
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| 7036 | 5' | -54.7 | NC_001876.1 | + | 22924 | 0.71 | 0.302348 |
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Target: 5'- gGGAcCUGAACggUGGGUgUGGGUGGUCu -3' miRNA: 3'- aCCUcGAUUUGa-ACCCG-GUCCACCAG- -5' |
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| 7036 | 5' | -54.7 | NC_001876.1 | + | 8522 | 0.97 | 0.003573 |
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Target: 5'- gUGGAGCUAAACUUGGGCCAGGU-GUCa -3' miRNA: 3'- -ACCUCGAUUUGAACCCGGUCCAcCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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