Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7037 | 5' | -59.6 | NC_001876.1 | + | 7704 | 0.66 | 0.330504 |
Target: 5'- aAGGGaaGGCGCGGCcugucagcGCGGGC-CGGGa -3' miRNA: 3'- aUCCCggCUGCGCCG--------CGUCUGuGUUU- -5' |
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7037 | 5' | -59.6 | NC_001876.1 | + | 18008 | 0.66 | 0.32233 |
Target: 5'- -cGGGCaCGAaGCGGagcuGCAGACGCu-- -3' miRNA: 3'- auCCCG-GCUgCGCCg---CGUCUGUGuuu -5' |
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7037 | 5' | -59.6 | NC_001876.1 | + | 14621 | 0.66 | 0.306438 |
Target: 5'- cGGuGGCCGACacaaucgagGCGGUGguGGCugAc- -3' miRNA: 3'- aUC-CCGGCUG---------CGCCGCguCUGugUuu -5' |
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7037 | 5' | -59.6 | NC_001876.1 | + | 21802 | 0.66 | 0.306438 |
Target: 5'- --cGGCCGGCaaaGCGGCGUcaaAGugACAGc -3' miRNA: 3'- aucCCGGCUG---CGCCGCG---UCugUGUUu -5' |
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7037 | 5' | -59.6 | NC_001876.1 | + | 8504 | 0.66 | 0.298719 |
Target: 5'- cGGGGCUgaggcggagGugGUGGUGCAGAUAa--- -3' miRNA: 3'- aUCCCGG---------CugCGCCGCGUCUGUguuu -5' |
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7037 | 5' | -59.6 | NC_001876.1 | + | 16092 | 0.66 | 0.298719 |
Target: 5'- cGGGGCUuGCGCGGCacgaccacugccGCGGGCAaAGAg -3' miRNA: 3'- aUCCCGGcUGCGCCG------------CGUCUGUgUUU- -5' |
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7037 | 5' | -59.6 | NC_001876.1 | + | 9372 | 0.66 | 0.291152 |
Target: 5'- -cGGGCaCGuuGCGGCGCugcGGGgGCAGGg -3' miRNA: 3'- auCCCG-GCugCGCCGCG---UCUgUGUUU- -5' |
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7037 | 5' | -59.6 | NC_001876.1 | + | 108 | 0.68 | 0.235973 |
Target: 5'- gUGGGGCgGAgCGCGuCGCGGAgGCGGc -3' miRNA: 3'- -AUCCCGgCU-GCGCcGCGUCUgUGUUu -5' |
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7037 | 5' | -59.6 | NC_001876.1 | + | 34339 | 0.68 | 0.235973 |
Target: 5'- gUGGGGCgGAgCGCGuCGCGGAgGCGGc -3' miRNA: 3'- -AUCCCGgCU-GCGCcGCGUCUgUGUUu -5' |
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7037 | 5' | -59.6 | NC_001876.1 | + | 16503 | 0.7 | 0.174685 |
Target: 5'- cAGuGGCCGGCGcCGGCGCcgaacAGGCuuGCGAGg -3' miRNA: 3'- aUC-CCGGCUGC-GCCGCG-----UCUG--UGUUU- -5' |
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7037 | 5' | -59.6 | NC_001876.1 | + | 161 | 0.71 | 0.127986 |
Target: 5'- gGGGGCgGcGCGCGGgGCGGcGCGCGGGg -3' miRNA: 3'- aUCCCGgC-UGCGCCgCGUC-UGUGUUU- -5' |
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7037 | 5' | -59.6 | NC_001876.1 | + | 34286 | 0.71 | 0.127986 |
Target: 5'- gGGGGCgGcGCGCGGgGCGGcGCGCGGGg -3' miRNA: 3'- aUCCCGgC-UGCGCCgCGUC-UGUGUUU- -5' |
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7037 | 5' | -59.6 | NC_001876.1 | + | 15706 | 0.72 | 0.107655 |
Target: 5'- cGGGGCCGGCuccGUGGCcgaaguGCAGAUGCGAGa -3' miRNA: 3'- aUCCCGGCUG---CGCCG------CGUCUGUGUUU- -5' |
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7037 | 5' | -59.6 | NC_001876.1 | + | 14783 | 0.76 | 0.06343 |
Target: 5'- -uGGGCCGgcGCGCGGCcgcuaucgcaGCAGACGCGGc -3' miRNA: 3'- auCCCGGC--UGCGCCG----------CGUCUGUGUUu -5' |
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7037 | 5' | -59.6 | NC_001876.1 | + | 15347 | 1.04 | 0.000365 |
Target: 5'- gUAGGGCCGACGCGGCGCAGACACAAAu -3' miRNA: 3'- -AUCCCGGCUGCGCCGCGUCUGUGUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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