Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7039 | 3' | -58.4 | NC_001876.1 | + | 20202 | 0.66 | 0.350847 |
Target: 5'- -gGGGGUagGGguGGCCcuCGCGCGGaGCg -3' miRNA: 3'- uaUCCCG--UCguUCGGc-GCGCGUCaUG- -5' |
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7039 | 3' | -58.4 | NC_001876.1 | + | 11083 | 0.67 | 0.309132 |
Target: 5'- --cGGGCcccGGCAaagcGGCCGC-CGCGGUcGCg -3' miRNA: 3'- uauCCCG---UCGU----UCGGCGcGCGUCA-UG- -5' |
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7039 | 3' | -58.4 | NC_001876.1 | + | 14544 | 0.67 | 0.306753 |
Target: 5'- cUGGGGCAGCcgcaccggucggguGGGUCGCcGCGCAa--- -3' miRNA: 3'- uAUCCCGUCG--------------UUCGGCG-CGCGUcaug -5' |
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7039 | 3' | -58.4 | NC_001876.1 | + | 18175 | 0.68 | 0.257251 |
Target: 5'- -gGGGGCAaaggaguuGUAGGCCGUGcCGCuGUAa -3' miRNA: 3'- uaUCCCGU--------CGUUCGGCGC-GCGuCAUg -5' |
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7039 | 3' | -58.4 | NC_001876.1 | + | 25758 | 0.69 | 0.23665 |
Target: 5'- -gAGGGCGGCGagaaucgugucggAGCUGCagaGCAGUAUu -3' miRNA: 3'- uaUCCCGUCGU-------------UCGGCGcg-CGUCAUG- -5' |
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7039 | 3' | -58.4 | NC_001876.1 | + | 14769 | 0.7 | 0.195748 |
Target: 5'- -gAGGGCccgcagGGUggGCCgGCGCGCGGccGCu -3' miRNA: 3'- uaUCCCG------UCGuuCGG-CGCGCGUCa-UG- -5' |
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7039 | 3' | -58.4 | NC_001876.1 | + | 14328 | 0.72 | 0.143309 |
Target: 5'- -aGGGGCAGgAucuucGCCGCGCGuCAGUc- -3' miRNA: 3'- uaUCCCGUCgUu----CGGCGCGC-GUCAug -5' |
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7039 | 3' | -58.4 | NC_001876.1 | + | 16577 | 0.73 | 0.110311 |
Target: 5'- --cGGGCGGC-GGCCGCcuGCGCGGggGCu -3' miRNA: 3'- uauCCCGUCGuUCGGCG--CGCGUCa-UG- -5' |
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7039 | 3' | -58.4 | NC_001876.1 | + | 16299 | 0.74 | 0.100693 |
Target: 5'- --cGGGCAGCAgcgccgcGGCCGCG-GCAGcGCu -3' miRNA: 3'- uauCCCGUCGU-------UCGGCGCgCGUCaUG- -5' |
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7039 | 3' | -58.4 | NC_001876.1 | + | 152 | 0.81 | 0.028388 |
Target: 5'- -gAGGGCGGCGggGGCgGCGCGCGGgGCg -3' miRNA: 3'- uaUCCCGUCGU--UCGgCGCGCGUCaUG- -5' |
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7039 | 3' | -58.4 | NC_001876.1 | + | 34295 | 0.81 | 0.028388 |
Target: 5'- -gAGGGCGGCGggGGCgGCGCGCGGgGCg -3' miRNA: 3'- uaUCCCGUCGU--UCGgCGCGCGUCaUG- -5' |
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7039 | 3' | -58.4 | NC_001876.1 | + | 16139 | 1.08 | 0.000217 |
Target: 5'- aAUAGGGCAGCAAGCCGCGCGCAGUACg -3' miRNA: 3'- -UAUCCCGUCGUUCGGCGCGCGUCAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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