Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7039 | 5' | -60.7 | NC_001876.1 | + | 112 | 0.66 | 0.344382 |
Target: 5'- -gGCGGagcGCGuCGCGGaGGCggcgGCGCuGGg -3' miRNA: 3'- cgUGCCa--CGU-GCGCC-CCGaa--CGCG-CC- -5' |
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7039 | 5' | -60.7 | NC_001876.1 | + | 34335 | 0.66 | 0.344382 |
Target: 5'- -gGCGGagcGCGuCGCGGaGGCggcgGCGCuGGg -3' miRNA: 3'- cgUGCCa--CGU-GCGCC-CCGaa--CGCG-CC- -5' |
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7039 | 5' | -60.7 | NC_001876.1 | + | 17632 | 0.66 | 0.336247 |
Target: 5'- -gGCGGUGCuauUGagGGGGCgcGCaGCGGu -3' miRNA: 3'- cgUGCCACGu--GCg-CCCCGaaCG-CGCC- -5' |
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7039 | 5' | -60.7 | NC_001876.1 | + | 26315 | 0.67 | 0.305121 |
Target: 5'- gGCGCGGU-CugGa-GGGCaggGCGUGGa -3' miRNA: 3'- -CGUGCCAcGugCgcCCCGaa-CGCGCC- -5' |
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7039 | 5' | -60.7 | NC_001876.1 | + | 15185 | 0.68 | 0.256066 |
Target: 5'- aUACGGUGCuGCGCGuucucgccuGGGCcguUUGCGCu- -3' miRNA: 3'- cGUGCCACG-UGCGC---------CCCG---AACGCGcc -5' |
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7039 | 5' | -60.7 | NC_001876.1 | + | 26946 | 0.68 | 0.231048 |
Target: 5'- aGCGCGGggGagACGCGGagggugagcGGCUUGC-CGGg -3' miRNA: 3'- -CGUGCCa-Cg-UGCGCC---------CCGAACGcGCC- -5' |
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7039 | 5' | -60.7 | NC_001876.1 | + | 783 | 0.7 | 0.172727 |
Target: 5'- uCACgGGUGaCACagaaGCGGGGacagGCGCGGg -3' miRNA: 3'- cGUG-CCAC-GUG----CGCCCCgaa-CGCGCC- -5' |
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7039 | 5' | -60.7 | NC_001876.1 | + | 16573 | 0.71 | 0.163648 |
Target: 5'- cGCGCGG-GCG-GCGGccGCcUGCGCGGg -3' miRNA: 3'- -CGUGCCaCGUgCGCCc-CGaACGCGCC- -5' |
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7039 | 5' | -60.7 | NC_001876.1 | + | 158 | 0.87 | 0.009452 |
Target: 5'- gGCGgGGgcgGCGCGCGGGGCggcGCGCGGg -3' miRNA: 3'- -CGUgCCa--CGUGCGCCCCGaa-CGCGCC- -5' |
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7039 | 5' | -60.7 | NC_001876.1 | + | 34289 | 0.87 | 0.009452 |
Target: 5'- gGCGgGGgcgGCGCGCGGGGCggcGCGCGGg -3' miRNA: 3'- -CGUgCCa--CGUGCGCCCCGaa-CGCGCC- -5' |
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7039 | 5' | -60.7 | NC_001876.1 | + | 16105 | 1.11 | 0.000129 |
Target: 5'- aGCACGGUGCACGCGGGGCUUGCGCGGc -3' miRNA: 3'- -CGUGCCACGUGCGCCCCGAACGCGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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