Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7040 | 5' | -60.3 | NC_001876.1 | + | 27180 | 0.66 | 0.348401 |
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Target: 5'- cCCGGCcucGCCUGuGUgg-CGCCCACuGCUu -3' miRNA: 3'- -GGUCG---UGGGC-CAacaGCGGGUGcCGA- -5' |
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| 7040 | 5' | -60.3 | NC_001876.1 | + | 22319 | 0.66 | 0.316265 |
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Target: 5'- uCCAGCaagGCCCGGcugGcCGCCgGCagGGCg -3' miRNA: 3'- -GGUCG---UGGGCCaa-CaGCGGgUG--CCGa -5' |
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| 7040 | 5' | -60.3 | NC_001876.1 | + | 11590 | 0.67 | 0.286415 |
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Target: 5'- gCCGGCccccgGCCCGGUg--CGCUCGCGcuGCg -3' miRNA: 3'- -GGUCG-----UGGGCCAacaGCGGGUGC--CGa -5' |
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| 7040 | 5' | -60.3 | NC_001876.1 | + | 19208 | 0.69 | 0.227446 |
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Target: 5'- -aGGCACgCGGUgGUCGCCCGauGUg -3' miRNA: 3'- ggUCGUGgGCCAaCAGCGGGUgcCGa -5' |
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| 7040 | 5' | -60.3 | NC_001876.1 | + | 16276 | 0.69 | 0.220989 |
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Target: 5'- gCAGCGCUgaggCGGggaGUCGCCCugcggguACGGCg -3' miRNA: 3'- gGUCGUGG----GCCaa-CAGCGGG-------UGCCGa -5' |
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| 7040 | 5' | -60.3 | NC_001876.1 | + | 16003 | 0.7 | 0.179085 |
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Target: 5'- cUCAGCACCCaaacUGacucuUUGCCCGCGGCa -3' miRNA: 3'- -GGUCGUGGGcca-AC-----AGCGGGUGCCGa -5' |
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| 7040 | 5' | -60.3 | NC_001876.1 | + | 18081 | 1.09 | 0.000185 |
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Target: 5'- uCCAGCACCCGGUUGUCGCCCACGGCUa -3' miRNA: 3'- -GGUCGUGGGCCAACAGCGGGUGCCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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