Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7041 | 3' | -67.4 | NC_001876.1 | + | 10107 | 0.66 | 0.13094 |
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Target: 5'- aCCGC-CUgagucaaCGCUCgcGCCGGCGGCCGc -3' miRNA: 3'- cGGCGaGG-------GCGGGa-CGGCCGCCGGUc -5' |
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| 7041 | 3' | -67.4 | NC_001876.1 | + | 13004 | 0.67 | 0.117951 |
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Target: 5'- aGCCaccaCCGCCUcauaaGCCGGCGGCgGGa -3' miRNA: 3'- -CGGcgagGGCGGGa----CGGCCGCCGgUC- -5' |
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| 7041 | 3' | -67.4 | NC_001876.1 | + | 11024 | 0.68 | 0.103052 |
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Target: 5'- cGCgGCggCCGCUUUGCCGG-GGCCc- -3' miRNA: 3'- -CGgCGagGGCGGGACGGCCgCCGGuc -5' |
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| 7041 | 3' | -67.4 | NC_001876.1 | + | 14832 | 0.68 | 0.097604 |
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Target: 5'- gGCCGCgcgccggCCCaCCCUGCgGGCccucGCCAc -3' miRNA: 3'- -CGGCGa------GGGcGGGACGgCCGc---CGGUc -5' |
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| 7041 | 3' | -67.4 | NC_001876.1 | + | 17281 | 0.69 | 0.081495 |
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Target: 5'- cGCCGCcggcugacguucUCCUGCCCgcauccucaaagccGCCGGUGGCg-- -3' miRNA: 3'- -CGGCG------------AGGGCGGGa-------------CGGCCGCCGguc -5' |
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| 7041 | 3' | -67.4 | NC_001876.1 | + | 16621 | 0.72 | 0.043761 |
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Target: 5'- gGCCGCcgCCCGCgcgccuUCUG-CGGCGGCCGa -3' miRNA: 3'- -CGGCGa-GGGCG------GGACgGCCGCCGGUc -5' |
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| 7041 | 3' | -67.4 | NC_001876.1 | + | 1744 | 0.74 | 0.031231 |
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Target: 5'- cGCCGCUCuuGCUCUGCUGGUGuuCAu -3' miRNA: 3'- -CGGCGAGggCGGGACGGCCGCcgGUc -5' |
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| 7041 | 3' | -67.4 | NC_001876.1 | + | 22394 | 1.08 | 4.2e-05 |
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Target: 5'- cGCCGCUCCCGCCCUGCCGGCGGCCAGc -3' miRNA: 3'- -CGGCGAGGGCGGGACGGCCGCCGGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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