Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7043 | 3' | -54.8 | NC_001876.1 | + | 16899 | 0.66 | 0.569699 |
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Target: 5'- --cCGACCCAuGCUG--AGCCAGUGGu -3' miRNA: 3'- ucuGCUGGGUcUGAUggUCGGUCAUC- -5' |
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| 7043 | 3' | -54.8 | NC_001876.1 | + | 14609 | 0.66 | 0.569699 |
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Target: 5'- cGGCGACCCAcccgaccggugcGGCUGCC--CCAGgcGg -3' miRNA: 3'- uCUGCUGGGU------------CUGAUGGucGGUCauC- -5' |
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| 7043 | 3' | -54.8 | NC_001876.1 | + | 17174 | 0.68 | 0.460589 |
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Target: 5'- uGACGGCCCAGAUccaCGGCUccGUGGa -3' miRNA: 3'- uCUGCUGGGUCUGaugGUCGGu-CAUC- -5' |
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| 7043 | 3' | -54.8 | NC_001876.1 | + | 8341 | 0.69 | 0.400647 |
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Target: 5'- cGGugGACCguGGCUGCCAaGUCcGUGc -3' miRNA: 3'- -UCugCUGGguCUGAUGGU-CGGuCAUc -5' |
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| 7043 | 3' | -54.8 | NC_001876.1 | + | 15497 | 0.77 | 0.118163 |
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Target: 5'- gAGGCGGCCCAGgcccagcuGCUACCGGCU-GUGGc -3' miRNA: 3'- -UCUGCUGGGUC--------UGAUGGUCGGuCAUC- -5' |
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| 7043 | 3' | -54.8 | NC_001876.1 | + | 1922 | 1.09 | 0.000552 |
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Target: 5'- aAGACGACCCAGACUACCAGCCAGUAGa -3' miRNA: 3'- -UCUGCUGGGUCUGAUGGUCGGUCAUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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