Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7043 | 5' | -63.4 | NC_001876.1 | + | 8865 | 0.66 | 0.232256 |
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Target: 5'- cCCCAgCCCGGaaccgguagaGCUGGagaugcccccgCUGGAGGGGg -3' miRNA: 3'- cGGGUgGGGCC----------CGAUCg----------GACCUCCUC- -5' |
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| 7043 | 5' | -63.4 | NC_001876.1 | + | 16507 | 0.67 | 0.203988 |
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Target: 5'- gGCCgGCgCCGGcGCcgaacaGGCUUGcGAGGAGg -3' miRNA: 3'- -CGGgUGgGGCC-CGa-----UCGGAC-CUCCUC- -5' |
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| 7043 | 5' | -63.4 | NC_001876.1 | + | 13428 | 0.67 | 0.174049 |
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Target: 5'- -gUUACCCuCGGGC-AGCCUuaGGGGGAa -3' miRNA: 3'- cgGGUGGG-GCCCGaUCGGA--CCUCCUc -5' |
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| 7043 | 5' | -63.4 | NC_001876.1 | + | 26329 | 0.68 | 0.164973 |
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Target: 5'- uCCUAugcCCCCGuGGCgcGGUCUGGAGGGc -3' miRNA: 3'- cGGGU---GGGGC-CCGa-UCGGACCUCCUc -5' |
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| 7043 | 5' | -63.4 | NC_001876.1 | + | 2019 | 0.69 | 0.129203 |
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Target: 5'- aGCCUcCUCCaGGCUAGCCcGGGGuGGGc -3' miRNA: 3'- -CGGGuGGGGcCCGAUCGGaCCUC-CUC- -5' |
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| 7043 | 5' | -63.4 | NC_001876.1 | + | 1957 | 1.1 | 6.8e-05 |
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Target: 5'- aGCCCACCCCGGGCUAGCCUGGAGGAGg -3' miRNA: 3'- -CGGGUGGGGCCCGAUCGGACCUCCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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