miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
7045 5' -52.7 NC_001876.1 + 12380 0.66 0.765233
Target:  5'- uGUGGGAUGauuuggacaGCGCGcUC-CUaCGCGCg -3'
miRNA:   3'- gCAUUCUGCg--------CGCGUuAGaGA-GCGCG- -5'
7045 5' -52.7 NC_001876.1 + 12467 0.67 0.687808
Target:  5'- ----cGGCGCcCGC-GUCUCgCGCGCa -3'
miRNA:   3'- gcauuCUGCGcGCGuUAGAGaGCGCG- -5'
7045 5' -52.7 NC_001876.1 + 3429 0.68 0.618975
Target:  5'- --cAGGACGaCGCGCuccCUCggCGUGCa -3'
miRNA:   3'- gcaUUCUGC-GCGCGuuaGAGa-GCGCG- -5'
7045 5' -52.7 NC_001876.1 + 156 0.68 0.607481
Target:  5'- gCGgcgGGGGCgGCGCGCGGggcggCGCGCg -3'
miRNA:   3'- -GCa--UUCUG-CGCGCGUUagagaGCGCG- -5'
7045 5' -52.7 NC_001876.1 + 34291 0.68 0.607481
Target:  5'- gCGgcgGGGGCgGCGCGCGGggcggCGCGCg -3'
miRNA:   3'- -GCa--UUCUG-CGCGCGUUagagaGCGCG- -5'
7045 5' -52.7 NC_001876.1 + 1444 0.71 0.442835
Target:  5'- cCGUAGGGCGCGC-CAAagucaacaugacUCacauuuccUCUUGCGCg -3'
miRNA:   3'- -GCAUUCUGCGCGcGUU------------AG--------AGAGCGCG- -5'
7045 5' -52.7 NC_001876.1 + 9909 1.12 0.000638
Target:  5'- cCGUAAGACGCGCGCAAUCUCUCGCGCu -3'
miRNA:   3'- -GCAUUCUGCGCGCGUUAGAGAGCGCG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.