Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7045 | 5' | -52.7 | NC_001876.1 | + | 12380 | 0.66 | 0.765233 |
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Target: 5'- uGUGGGAUGauuuggacaGCGCGcUC-CUaCGCGCg -3' miRNA: 3'- gCAUUCUGCg--------CGCGUuAGaGA-GCGCG- -5' |
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| 7045 | 5' | -52.7 | NC_001876.1 | + | 12467 | 0.67 | 0.687808 |
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Target: 5'- ----cGGCGCcCGC-GUCUCgCGCGCa -3' miRNA: 3'- gcauuCUGCGcGCGuUAGAGaGCGCG- -5' |
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| 7045 | 5' | -52.7 | NC_001876.1 | + | 3429 | 0.68 | 0.618975 |
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Target: 5'- --cAGGACGaCGCGCuccCUCggCGUGCa -3' miRNA: 3'- gcaUUCUGC-GCGCGuuaGAGa-GCGCG- -5' |
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| 7045 | 5' | -52.7 | NC_001876.1 | + | 156 | 0.68 | 0.607481 |
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Target: 5'- gCGgcgGGGGCgGCGCGCGGggcggCGCGCg -3' miRNA: 3'- -GCa--UUCUG-CGCGCGUUagagaGCGCG- -5' |
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| 7045 | 5' | -52.7 | NC_001876.1 | + | 34291 | 0.68 | 0.607481 |
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Target: 5'- gCGgcgGGGGCgGCGCGCGGggcggCGCGCg -3' miRNA: 3'- -GCa--UUCUG-CGCGCGUUagagaGCGCG- -5' |
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| 7045 | 5' | -52.7 | NC_001876.1 | + | 1444 | 0.71 | 0.442835 |
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Target: 5'- cCGUAGGGCGCGC-CAAagucaacaugacUCacauuuccUCUUGCGCg -3' miRNA: 3'- -GCAUUCUGCGCGcGUU------------AG--------AGAGCGCG- -5' |
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| 7045 | 5' | -52.7 | NC_001876.1 | + | 9909 | 1.12 | 0.000638 |
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Target: 5'- cCGUAAGACGCGCGCAAUCUCUCGCGCu -3' miRNA: 3'- -GCAUUCUGCGCGCGUUAGAGAGCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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