Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7046 | 3' | -58.7 | NC_001876.1 | + | 23826 | 0.66 | 0.391261 |
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Target: 5'- cAGGC-UUGGCgcaCGUAGCCgU-GGCGAa -3' miRNA: 3'- -UCUGaAACCGa--GCGUCGGgGuCCGCU- -5' |
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| 7046 | 3' | -58.7 | NC_001876.1 | + | 16598 | 0.66 | 0.36417 |
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Target: 5'- gGGGCUUccuuccccuccUGGCUCccaucauuGCAGCCgCCAucGGCGc -3' miRNA: 3'- -UCUGAA-----------ACCGAG--------CGUCGG-GGU--CCGCu -5' |
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| 7046 | 3' | -58.7 | NC_001876.1 | + | 1390 | 0.66 | 0.355432 |
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Target: 5'- uGACUUUGGC--GC-GCCCUAcGGUGAc -3' miRNA: 3'- uCUGAAACCGagCGuCGGGGU-CCGCU- -5' |
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| 7046 | 3' | -58.7 | NC_001876.1 | + | 26969 | 0.68 | 0.290931 |
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Target: 5'- uGACUgcUUGGUggGCAGuUCCCAGcGCGGg -3' miRNA: 3'- uCUGA--AACCGagCGUC-GGGGUC-CGCU- -5' |
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| 7046 | 3' | -58.7 | NC_001876.1 | + | 14597 | 0.68 | 0.276308 |
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Target: 5'- cGACc--GGUgcgGCuGCCCCAGGCGGc -3' miRNA: 3'- uCUGaaaCCGag-CGuCGGGGUCCGCU- -5' |
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| 7046 | 3' | -58.7 | NC_001876.1 | + | 9295 | 0.69 | 0.235964 |
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Target: 5'- uGAUgc-GGUUUGCaaAGCCCCAGGCu- -3' miRNA: 3'- uCUGaaaCCGAGCG--UCGGGGUCCGcu -5' |
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| 7046 | 3' | -58.7 | NC_001876.1 | + | 11277 | 1.09 | 0.000215 |
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Target: 5'- gAGACUUUGGCUCGCAGCCCCAGGCGAa -3' miRNA: 3'- -UCUGAAACCGAGCGUCGGGGUCCGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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