Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7046 | 5' | -59.1 | NC_001876.1 | + | 21732 | 0.66 | 0.38696 |
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Target: 5'- uGCCAgaaagCCUgCaAAGCCuUGG-GCCCCu -3' miRNA: 3'- uCGGUa----GGA-GcUUCGGcACCaCGGGG- -5' |
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| 7046 | 5' | -59.1 | NC_001876.1 | + | 17302 | 0.67 | 0.32705 |
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Target: 5'- uGCCcgcAUCCUCaAAGCCGccggUGGcgguggugaccuUGCCCCc -3' miRNA: 3'- uCGG---UAGGAGcUUCGGC----ACC------------ACGGGG- -5' |
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| 7046 | 5' | -59.1 | NC_001876.1 | + | 14624 | 0.67 | 0.318284 |
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Target: 5'- uGGCCGaCacaaUCGAGGCgGUGGUggcugacGCCCg -3' miRNA: 3'- -UCGGUaGg---AGCUUCGgCACCA-------CGGGg -5' |
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| 7046 | 5' | -59.1 | NC_001876.1 | + | 17248 | 0.68 | 0.296026 |
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Target: 5'- cGCCcUCCUacgcCGAAGCgGUGGcugaGCgCCCg -3' miRNA: 3'- uCGGuAGGA----GCUUCGgCACCa---CG-GGG- -5' |
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| 7046 | 5' | -59.1 | NC_001876.1 | + | 17105 | 0.69 | 0.260527 |
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Target: 5'- cGCCGguaUCCaCGGAGCCGUGGaucuggGCCg- -3' miRNA: 3'- uCGGU---AGGaGCUUCGGCACCa-----CGGgg -5' |
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| 7046 | 5' | -59.1 | NC_001876.1 | + | 11145 | 0.73 | 0.136021 |
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Target: 5'- uGGCCGaagcgCCcCGAAGCC-UGG-GCCCCg -3' miRNA: 3'- -UCGGUa----GGaGCUUCGGcACCaCGGGG- -5' |
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| 7046 | 5' | -59.1 | NC_001876.1 | + | 25112 | 0.78 | 0.054105 |
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Target: 5'- cGCCcUCCUCGAGcucGCCG-GaGUGCCCCu -3' miRNA: 3'- uCGGuAGGAGCUU---CGGCaC-CACGGGG- -5' |
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| 7046 | 5' | -59.1 | NC_001876.1 | + | 11313 | 1.1 | 0.000174 |
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Target: 5'- gAGCCAUCCUCGAAGCCGUGGUGCCCCc -3' miRNA: 3'- -UCGGUAGGAGCUUCGGCACCACGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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