Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7048 | 3' | -55.9 | NC_001876.1 | + | 22365 | 0.66 | 0.565241 |
Target: 5'- ---gGGCCuUGCUGGAaGCCGACgaagUUGCu -3' miRNA: 3'- gacaCCGGcACGGCCU-UGGCUG----AAUG- -5' |
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7048 | 3' | -55.9 | NC_001876.1 | + | 16972 | 0.66 | 0.554094 |
Target: 5'- -cGUGcguGCCGaUGCCGGAccACUGGCUcaGCa -3' miRNA: 3'- gaCAC---CGGC-ACGGCCU--UGGCUGAa-UG- -5' |
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7048 | 3' | -55.9 | NC_001876.1 | + | 16504 | 0.77 | 0.112339 |
Target: 5'- -aGUGGCCGgcGCCGGcGCCGaacagGCUUGCg -3' miRNA: 3'- gaCACCGGCa-CGGCCuUGGC-----UGAAUG- -5' |
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7048 | 3' | -55.9 | NC_001876.1 | + | 15524 | 1.1 | 0.000383 |
Target: 5'- gCUGUGGCCGUGCCGGAACCGACUUACg -3' miRNA: 3'- -GACACCGGCACGGCCUUGGCUGAAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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