miRNA display CGI


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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
7048 3' -55.9 NC_001876.1 + 22365 0.66 0.565241
Target:  5'- ---gGGCCuUGCUGGAaGCCGACgaagUUGCu -3'
miRNA:   3'- gacaCCGGcACGGCCU-UGGCUG----AAUG- -5'
7048 3' -55.9 NC_001876.1 + 16972 0.66 0.554094
Target:  5'- -cGUGcguGCCGaUGCCGGAccACUGGCUcaGCa -3'
miRNA:   3'- gaCAC---CGGC-ACGGCCU--UGGCUGAa-UG- -5'
7048 3' -55.9 NC_001876.1 + 16504 0.77 0.112339
Target:  5'- -aGUGGCCGgcGCCGGcGCCGaacagGCUUGCg -3'
miRNA:   3'- gaCACCGGCa-CGGCCuUGGC-----UGAAUG- -5'
7048 3' -55.9 NC_001876.1 + 15524 1.1 0.000383
Target:  5'- gCUGUGGCCGUGCCGGAACCGACUUACg -3'
miRNA:   3'- -GACACCGGCACGGCCUUGGCUGAAUG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.