Results 1 - 20 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7048 | 5' | -56.4 | NC_001876.1 | + | 16217 | 0.66 | 0.511474 |
Target: 5'- cGGGCAGgCgCACaCCCGAGCU-CGuGUa -3' miRNA: 3'- -CUCGUUgGgGUG-GGGCUCGAuGUuCG- -5' |
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7048 | 5' | -56.4 | NC_001876.1 | + | 26537 | 0.66 | 0.500683 |
Target: 5'- uGAGCAgaaACCCCuucauGCUugUgGGGCUugAAGCg -3' miRNA: 3'- -CUCGU---UGGGG-----UGG--GgCUCGAugUUCG- -5' |
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7048 | 5' | -56.4 | NC_001876.1 | + | 161 | 0.66 | 0.500683 |
Target: 5'- -cGCGacgcGCUCCGCCCCacAGUUACGucAGCa -3' miRNA: 3'- cuCGU----UGGGGUGGGGc-UCGAUGU--UCG- -5' |
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7048 | 5' | -56.4 | NC_001876.1 | + | 34286 | 0.66 | 0.500683 |
Target: 5'- -cGCGacgcGCUCCGCCCCacAGUUACGucAGCa -3' miRNA: 3'- cuCGU----UGGGGUGGGGc-UCGAUGU--UCG- -5' |
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7048 | 5' | -56.4 | NC_001876.1 | + | 11289 | 0.66 | 0.500683 |
Target: 5'- -cGCAGCCCCAggcgaaCCG-GUUugGAGCc -3' miRNA: 3'- cuCGUUGGGGUgg----GGCuCGAugUUCG- -5' |
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7048 | 5' | -56.4 | NC_001876.1 | + | 17380 | 0.66 | 0.489993 |
Target: 5'- gGGGCAaggucACCaCCGCCaCCGgcGGCUuugaggauGCGGGCa -3' miRNA: 3'- -CUCGU-----UGG-GGUGG-GGC--UCGA--------UGUUCG- -5' |
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7048 | 5' | -56.4 | NC_001876.1 | + | 14609 | 0.66 | 0.479411 |
Target: 5'- cGGCGACCCa--CCCGAccggugcgGCUGCcccAGGCg -3' miRNA: 3'- cUCGUUGGGgugGGGCU--------CGAUG---UUCG- -5' |
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7048 | 5' | -56.4 | NC_001876.1 | + | 26850 | 0.66 | 0.468943 |
Target: 5'- aAGCugacguCCCUACCUgucaCGAGCccgGCAAGCc -3' miRNA: 3'- cUCGuu----GGGGUGGG----GCUCGa--UGUUCG- -5' |
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7048 | 5' | -56.4 | NC_001876.1 | + | 11161 | 0.66 | 0.468943 |
Target: 5'- aAGCcugGGCCCCGCCCCuuccaauccuAGuUUGCAGGCu -3' miRNA: 3'- cUCG---UUGGGGUGGGGc---------UC-GAUGUUCG- -5' |
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7048 | 5' | -56.4 | NC_001876.1 | + | 9000 | 0.67 | 0.448364 |
Target: 5'- aGAG--GCCgCGCCgCGGGCUgggGCAGGCu -3' miRNA: 3'- -CUCguUGGgGUGGgGCUCGA---UGUUCG- -5' |
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7048 | 5' | -56.4 | NC_001876.1 | + | 2840 | 0.67 | 0.428296 |
Target: 5'- -uGUAGCCCUGCCCCaGuguGCUuucuACAAGUc -3' miRNA: 3'- cuCGUUGGGGUGGGG-Cu--CGA----UGUUCG- -5' |
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7048 | 5' | -56.4 | NC_001876.1 | + | 24401 | 0.67 | 0.408769 |
Target: 5'- cGAcCAACCCCGCCCUGcucAGCgagaccCAAGUu -3' miRNA: 3'- -CUcGUUGGGGUGGGGC---UCGau----GUUCG- -5' |
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7048 | 5' | -56.4 | NC_001876.1 | + | 15498 | 0.67 | 0.408769 |
Target: 5'- aGGCGGCCCagGCCCagcuGCUACcGGCu -3' miRNA: 3'- cUCGUUGGGg-UGGGgcu-CGAUGuUCG- -5' |
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7048 | 5' | -56.4 | NC_001876.1 | + | 16217 | 0.67 | 0.408769 |
Target: 5'- aGGGCGAcuCCCCGCCUCaGcGCUGCcgcGGCc -3' miRNA: 3'- -CUCGUU--GGGGUGGGG-CuCGAUGu--UCG- -5' |
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7048 | 5' | -56.4 | NC_001876.1 | + | 16625 | 0.67 | 0.408769 |
Target: 5'- aGGCGGCCgCCGCCCgCGcgccuucugcGGCggcCGAGCa -3' miRNA: 3'- cUCGUUGG-GGUGGG-GC----------UCGau-GUUCG- -5' |
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7048 | 5' | -56.4 | NC_001876.1 | + | 13205 | 0.68 | 0.389806 |
Target: 5'- uAGCAACCaCAgCaUCGAGCUagACGAGCg -3' miRNA: 3'- cUCGUUGGgGUgG-GGCUCGA--UGUUCG- -5' |
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7048 | 5' | -56.4 | NC_001876.1 | + | 12901 | 0.68 | 0.380543 |
Target: 5'- gGGGCGauGCUCCAgCCCGAaCUGCA-GCc -3' miRNA: 3'- -CUCGU--UGGGGUgGGGCUcGAUGUuCG- -5' |
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7048 | 5' | -56.4 | NC_001876.1 | + | 11352 | 0.68 | 0.362464 |
Target: 5'- uGGCuccaAACCgguUCGCCUgGGGCUGCGAGCc -3' miRNA: 3'- cUCG----UUGG---GGUGGGgCUCGAUGUUCG- -5' |
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7048 | 5' | -56.4 | NC_001876.1 | + | 7775 | 0.68 | 0.35365 |
Target: 5'- aAGC-AUCCCGgCCCGcGCUgACAGGCc -3' miRNA: 3'- cUCGuUGGGGUgGGGCuCGA-UGUUCG- -5' |
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7048 | 5' | -56.4 | NC_001876.1 | + | 19346 | 0.69 | 0.311885 |
Target: 5'- aAGCGGCCgUugCCCaGcAGCUGgGAGCg -3' miRNA: 3'- cUCGUUGGgGugGGG-C-UCGAUgUUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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