Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7048 | 5' | -56.4 | NC_001876.1 | + | 17380 | 0.66 | 0.489993 |
Target: 5'- gGGGCAaggucACCaCCGCCaCCGgcGGCUuugaggauGCGGGCa -3' miRNA: 3'- -CUCGU-----UGG-GGUGG-GGC--UCGA--------UGUUCG- -5' |
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7048 | 5' | -56.4 | NC_001876.1 | + | 26537 | 0.66 | 0.500683 |
Target: 5'- uGAGCAgaaACCCCuucauGCUugUgGGGCUugAAGCg -3' miRNA: 3'- -CUCGU---UGGGG-----UGG--GgCUCGAugUUCG- -5' |
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7048 | 5' | -56.4 | NC_001876.1 | + | 161 | 0.66 | 0.500683 |
Target: 5'- -cGCGacgcGCUCCGCCCCacAGUUACGucAGCa -3' miRNA: 3'- cuCGU----UGGGGUGGGGc-UCGAUGU--UCG- -5' |
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7048 | 5' | -56.4 | NC_001876.1 | + | 16217 | 0.66 | 0.511474 |
Target: 5'- cGGGCAGgCgCACaCCCGAGCU-CGuGUa -3' miRNA: 3'- -CUCGUUgGgGUG-GGGCUCGAuGUuCG- -5' |
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7048 | 5' | -56.4 | NC_001876.1 | + | 2272 | 0.73 | 0.177206 |
Target: 5'- uAGUAGCCCCAuUUCCGAGCacAUAGGCg -3' miRNA: 3'- cUCGUUGGGGU-GGGGCUCGa-UGUUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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