Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7049 | 5' | -57.6 | NC_001876.1 | + | 16334 | 0.66 | 0.447318 |
Target: 5'- gGCCGCUUgGCGgaugCUagggugaUAGCG-CACGCCGg -3' miRNA: 3'- -UGGCGGA-CGUa---GG-------AUCGUaGUGCGGC- -5' |
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7049 | 5' | -57.6 | NC_001876.1 | + | 16585 | 0.66 | 0.422541 |
Target: 5'- gGCCGCCUGCgcGggggcuuccuuccccUCCUGGCucccAUCAUugcaGCCGc -3' miRNA: 3'- -UGGCGGACG--U---------------AGGAUCG----UAGUG----CGGC- -5' |
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7049 | 5' | -57.6 | NC_001876.1 | + | 26340 | 0.67 | 0.399547 |
Target: 5'- aACUGCCUGgAUCCUAuGCccccGUgGCGCgGu -3' miRNA: 3'- -UGGCGGACgUAGGAU-CG----UAgUGCGgC- -5' |
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7049 | 5' | -57.6 | NC_001876.1 | + | 14724 | 0.67 | 0.354297 |
Target: 5'- cACUGcCCUGCAgcgacggguUCgUAGCGU-ACGCCGa -3' miRNA: 3'- -UGGC-GGACGU---------AGgAUCGUAgUGCGGC- -5' |
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7049 | 5' | -57.6 | NC_001876.1 | + | 33404 | 0.67 | 0.354297 |
Target: 5'- cACCGCCUuucgacgugaGCAUUCUGGCAcagUCuuCGCUa -3' miRNA: 3'- -UGGCGGA----------CGUAGGAUCGU---AGu-GCGGc -5' |
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7049 | 5' | -57.6 | NC_001876.1 | + | 33548 | 0.67 | 0.354297 |
Target: 5'- cACCGCCUuucgacgugaGCAUUCUGGCAcagUCuuCGCUa -3' miRNA: 3'- -UGGCGGA----------CGUAGGAUCGU---AGu-GCGGc -5' |
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7049 | 5' | -57.6 | NC_001876.1 | + | 14868 | 0.68 | 0.343139 |
Target: 5'- cGCCGCCauggccauggccGCGUCUgcugcgaUAGCGgccgCGCGCCGg -3' miRNA: 3'- -UGGCGGa-----------CGUAGG-------AUCGUa---GUGCGGC- -5' |
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7049 | 5' | -57.6 | NC_001876.1 | + | 14157 | 0.69 | 0.297244 |
Target: 5'- gGCCcCCUGCG-CCUAcCAUCACGUCc -3' miRNA: 3'- -UGGcGGACGUaGGAUcGUAGUGCGGc -5' |
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7049 | 5' | -57.6 | NC_001876.1 | + | 14717 | 0.69 | 0.282308 |
Target: 5'- cGCCGUCUGCGgcgCCgguacuggcgGGCGUCA-GCCa -3' miRNA: 3'- -UGGCGGACGUa--GGa---------UCGUAGUgCGGc -5' |
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7049 | 5' | -57.6 | NC_001876.1 | + | 9857 | 0.69 | 0.275067 |
Target: 5'- gGCCGCggGCAucUCCUGGaUGUCGCGUCa -3' miRNA: 3'- -UGGCGgaCGU--AGGAUC-GUAGUGCGGc -5' |
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7049 | 5' | -57.6 | NC_001876.1 | + | 20881 | 0.69 | 0.267273 |
Target: 5'- cGCCGCCUGCggucuGUUCUGcuGCAUguuccucCACGCCu -3' miRNA: 3'- -UGGCGGACG-----UAGGAU--CGUA-------GUGCGGc -5' |
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7049 | 5' | -57.6 | NC_001876.1 | + | 10108 | 0.72 | 0.183611 |
Target: 5'- uACCGCCUGaGUCaacGC-UCGCGCCGg -3' miRNA: 3'- -UGGCGGACgUAGgauCGuAGUGCGGC- -5' |
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7049 | 5' | -57.6 | NC_001876.1 | + | 16257 | 0.72 | 0.164226 |
Target: 5'- uGCUGCCcgGCGUgcgcuaucacCCUAGCAUC-CGCCa -3' miRNA: 3'- -UGGCGGa-CGUA----------GGAUCGUAGuGCGGc -5' |
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7049 | 5' | -57.6 | NC_001876.1 | + | 16105 | 1.08 | 0.00031 |
Target: 5'- uACCGCCUGCAUCCUAGCAUCACGCCGa -3' miRNA: 3'- -UGGCGGACGUAGGAUCGUAGUGCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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