miRNA display CGI


Results 1 - 16 of 16 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
7050 3' -66.9 NC_001876.1 + 12986 0.66 0.154137
Target:  5'- aGCCgGCGGCgggacaGCGGaauugGCUGCaGUUCGGg -3'
miRNA:   3'- -CGG-CGCCGg-----CGCCg----CGACGaCGGGCC- -5'
7050 3' -66.9 NC_001876.1 + 10087 0.66 0.150141
Target:  5'- cGCCgGCGGCCGCaGCaggaacccccGCcaCUGCCgCGGa -3'
miRNA:   3'- -CGG-CGCCGGCGcCG----------CGacGACGG-GCC- -5'
7050 3' -66.9 NC_001876.1 + 34292 0.66 0.14624
Target:  5'- gGCgGCGGggGCGGCGCgcgggGCggcGCgCGGg -3'
miRNA:   3'- -CGgCGCCggCGCCGCGa----CGa--CGgGCC- -5'
7050 3' -66.9 NC_001876.1 + 155 0.66 0.14624
Target:  5'- gGCgGCGGggGCGGCGCgcgggGCggcGCgCGGg -3'
miRNA:   3'- -CGgCGCCggCGCCGCGa----CGa--CGgGCC- -5'
7050 3' -66.9 NC_001876.1 + 11014 0.67 0.135086
Target:  5'- cUCGCGcGaCCGCGGCgGCcGCuuUGCCgGGg -3'
miRNA:   3'- cGGCGC-C-GGCGCCG-CGaCG--ACGGgCC- -5'
7050 3' -66.9 NC_001876.1 + 17317 0.67 0.131546
Target:  5'- aGCCGcCGGUgGCGGUGgUGaccuUGCCCc- -3'
miRNA:   3'- -CGGC-GCCGgCGCCGCgACg---ACGGGcc -5'
7050 3' -66.9 NC_001876.1 + 22894 0.68 0.115095
Target:  5'- gGCCGCGaa-GCGGCuucuggcuccGCUGCUGCUaccgCGGg -3'
miRNA:   3'- -CGGCGCcggCGCCG----------CGACGACGG----GCC- -5'
7050 3' -66.9 NC_001876.1 + 16256 0.68 0.109068
Target:  5'- cGCCcuGCGGguaCgGCGGCGacgGCggcGCCCGGa -3'
miRNA:   3'- -CGG--CGCC---GgCGCCGCga-CGa--CGGGCC- -5'
7050 3' -66.9 NC_001876.1 + 14622 0.68 0.105311
Target:  5'- aGCUGCGGUugCGCGGCgacccacccgaccgGUgcgGCUGCCCc- -3'
miRNA:   3'- -CGGCGCCG--GCGCCG--------------CGa--CGACGGGcc -5'
7050 3' -66.9 NC_001876.1 + 11064 0.69 0.090231
Target:  5'- cGCCGCGGUCGC-GCGaguugGCggaGCCCaGGu -3'
miRNA:   3'- -CGGCGCCGGCGcCGCga---CGa--CGGG-CC- -5'
7050 3' -66.9 NC_001876.1 + 25541 0.69 0.083141
Target:  5'- uGUCGCGGCCcuCGGUGCuUGCgucgucgGCCUGa -3'
miRNA:   3'- -CGGCGCCGGc-GCCGCG-ACGa------CGGGCc -5'
7050 3' -66.9 NC_001876.1 + 10255 0.69 0.083141
Target:  5'- cGCUGCGGUCGCGGaauaaGUU-CU-CCCGGg -3'
miRNA:   3'- -CGGCGCCGGCGCCg----CGAcGAcGGGCC- -5'
7050 3' -66.9 NC_001876.1 + 9816 0.7 0.080898
Target:  5'- cCCGCGGCCgaaaugGCGGCcCUgGCguacucccggGCCCGGu -3'
miRNA:   3'- cGGCGCCGG------CGCCGcGA-CGa---------CGGGCC- -5'
7050 3' -66.9 NC_001876.1 + 10037 0.72 0.049204
Target:  5'- uGCUGCGGCCGcCGGCGCgagcGUUGaCUCaGGc -3'
miRNA:   3'- -CGGCGCCGGC-GCCGCGa---CGAC-GGG-CC- -5'
7050 3' -66.9 NC_001876.1 + 16289 0.81 0.010145
Target:  5'- cGCCGCGGCCGCGGCaGC-GCUGaggCGGg -3'
miRNA:   3'- -CGGCGCCGGCGCCG-CGaCGACgg-GCC- -5'
7050 3' -66.9 NC_001876.1 + 16240 1.09 0.000042
Target:  5'- uGCCGCGGCCGCGGCGCUGCUGCCCGGc -3'
miRNA:   3'- -CGGCGCCGGCGCCGCGACGACGGGCC- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.