Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7058 | 3' | -54.4 | NC_001876.1 | + | 22385 | 0.66 | 0.581255 |
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Target: 5'- cGCCCUGC-CGGCGgccagccGGGCCUugcuggaagccgacgAAGUUGc -3' miRNA: 3'- -CGGGACGaGUCGC-------UCUGGG---------------UUCAAUa -5' |
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| 7058 | 3' | -54.4 | NC_001876.1 | + | 17493 | 0.67 | 0.543524 |
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Target: 5'- cGCCCUGCUCGGC---ACCaGAGUc-- -3' miRNA: 3'- -CGGGACGAGUCGcucUGGgUUCAaua -5' |
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| 7058 | 3' | -54.4 | NC_001876.1 | + | 21819 | 0.67 | 0.521045 |
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Target: 5'- aGUCUUGUgguauguuuuUCAGCGAGggGCCCAAGg--- -3' miRNA: 3'- -CGGGACG----------AGUCGCUC--UGGGUUCaaua -5' |
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| 7058 | 3' | -54.4 | NC_001876.1 | + | 17079 | 0.69 | 0.425231 |
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Target: 5'- uGCCCgcauUGCUguGCcAGGCCCGAGa--- -3' miRNA: 3'- -CGGG----ACGAguCGcUCUGGGUUCaaua -5' |
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| 7058 | 3' | -54.4 | NC_001876.1 | + | 33422 | 0.69 | 0.386133 |
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Target: 5'- -aCCUGcCUCuGCGAGACCCAc----- -3' miRNA: 3'- cgGGAC-GAGuCGCUCUGGGUucaaua -5' |
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| 7058 | 3' | -54.4 | NC_001876.1 | + | 33566 | 0.69 | 0.386133 |
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Target: 5'- -aCCUGcCUCuGCGAGACCCAc----- -3' miRNA: 3'- cgGGAC-GAGuCGCUCUGGGUucaaua -5' |
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| 7058 | 3' | -54.4 | NC_001876.1 | + | 24411 | 1.08 | 0.000678 |
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Target: 5'- cGCCCUGCUCAGCGAGACCCAAGUUAUc -3' miRNA: 3'- -CGGGACGAGUCGCUCUGGGUUCAAUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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