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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7060 | 3' | -55.4 | NC_001876.1 | + | 16234 | 0.66 | 0.576017 |
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Target: 5'- cGGcgGcgCCCGGACGGcGGGCagGCg -3' miRNA: 3'- aCUaaCuaGGGUCUGUCaCCCGg-CGg -5' |
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| 7060 | 3' | -55.4 | NC_001876.1 | + | 9889 | 0.66 | 0.576017 |
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Target: 5'- ----cGggCCCGGG-AGUacgccaGGGCCGCCa -3' miRNA: 3'- acuaaCuaGGGUCUgUCA------CCCGGCGG- -5' |
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| 7060 | 3' | -55.4 | NC_001876.1 | + | 26074 | 0.66 | 0.552459 |
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Target: 5'- gUGggUGGUgCCCAccacggguuguuuGAUgaGGUGGGCgGCCc -3' miRNA: 3'- -ACuaACUA-GGGU-------------CUG--UCACCCGgCGG- -5' |
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| 7060 | 3' | -55.4 | NC_001876.1 | + | 16753 | 0.72 | 0.259895 |
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Target: 5'- aGAUUGAgcuuucuuuacacugUCCCuauuuguuGUGGGCCGCCu -3' miRNA: 3'- aCUAACU---------------AGGGucugu---CACCCGGCGG- -5' |
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| 7060 | 3' | -55.4 | NC_001876.1 | + | 25978 | 1.11 | 0.00037 |
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Target: 5'- cUGAUUGAUCCCAGACAGUGGGCCGCCc -3' miRNA: 3'- -ACUAACUAGGGUCUGUCACCCGGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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